- Catalog number
- EK0702
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 36 34 33 2.23 6.7% Sample 2 167 185 189 177 179 8.41 4.6% Sample 3 443 505 507 519 493 29.64 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 36 34 33 2.23 6.7% Sample 1 32 30 36 34 33 2.23 6.7% Sample 2 167 185 189 177 179 8.41 4.6% Sample 2 167 185 189 177 179 8.41 4.6% Sample 3 443 505 507 519 493 29.64 6% Sample 3 443 505 507 519 493 29.64 6%
- Synonyms
- Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40; CD40; TNFRSF5
- Gene Name
- CD40
- Gene ID
- 958
- UniProt
- P25942
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E21-R193
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- B-cells and in primary carcinomas.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Atherosclerosis, B Cells, Cardiovascular, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 167 443 32 177 404 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 167 443 32 177 404 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 167 443 32 177 404 Mean (pg/ml) 32 167 443 32 177 404 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6% CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6%
- Sensitivity
- <1 pg/mL
- Function
- Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 30619 MW
- Specificity
- Natural and recombinant human CD40
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily member 5
- Subcellular Location
- Isoform I: Cell membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 4 TNFR-Cys repeats.
- Protein Name
- Tumor necrosis factor receptor superfamily member 5
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
