Human CD56/NCAM-1 ELISA Kit PicoKine®

About this product

Human CD56/NCAM-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human NCAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0706

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 319 341 313 295 317 16.43 5.1% Sample 2 1085 1189 1030 1018 1080 67.54 6.2% Sample 3 5570 5244 5973 5762 5637 268.07 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 319 341 313 295 317 16.43 5.1% Sample 1 319 341 313 295 317 16.43 5.1% Sample 2 1085 1189 1030 1018 1080 67.54 6.2% Sample 2 1085 1189 1030 1018 1080 67.54 6.2% Sample 3 5570 5244 5973 5762 5637 268.07 4.7% Sample 3 5570 5244 5973 5762 5637 268.07 4.7%

Synonyms

Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD56; NCAM1; NCAM

Gene Name

NCAM1

Gene ID

4684

UniProt

P13591

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-G718

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Axonal Guidance Proteins, Cell Adhesion Proteins, Cell Type Marker, Developmental Biology, ECM Proteins, Growth and Development, Hematopoietic Progenitors, Membrane Proteins, Nervous System Development, Neural Signal Transduction, Neurology Process, Neuron Marker, Neuroscience, Organogenesis, Stem Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 319 1085 5570 313 1102 5558 Standard deviation 16.26 44.48 239.51 17.21 50.69 316.8 CV (%) 5.7%% 4.1% 4.3% 5.5% 4.6% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 319 1085 5570 313 1102 5558 Standard deviation 16.26 44.48 239.51 17.21 50.69 316.8 CV (%) 5.7%% 4.1% 4.3% 5.5% 4.6% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 319 1085 5570 313 1102 5558 Mean (pg/ml) 319 1085 5570 313 1102 5558 Standard deviation 16.26 44.48 239.51 17.21 50.69 316.8 Standard deviation 16.26 44.48 239.51 17.21 50.69 316.8 CV (%) 5.7%% 4.1% 4.3% 5.5% 4.6% 5.7% CV (%) 5.7%% 4.1% 4.3% 5.5% 4.6% 5.7%

Sensitivity

<10 pg/mL

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

94574 MW

Specificity

Natural and recombinant human CD56

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Neural cell adhesion molecule 1

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 2 fibronectin type-III domains.

Protein Name

Neural cell adhesion molecule 1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.095 0.155 0.241 0.432 0.76 1.3 1.838 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.095 0.155 0.241 0.432 0.76 1.3 1.838 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.095 0.155 0.241 0.432 0.76 1.3 1.838 O.D. 0.029 0.095 0.155 0.241 0.432 0.76 1.3 1.838

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.