- Catalog number
- EK0707
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 138 164 139 150 12.27 8.1% Sample 2 445 495 515 457 478 28.23 5.9% Sample 3 1656 1667 1402 1560 1571 106.21 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 138 164 139 150 12.27 8.1% Sample 1 162 138 164 139 150 12.27 8.1% Sample 2 445 495 515 457 478 28.23 5.9% Sample 2 445 495 515 457 478 28.23 5.9% Sample 3 1656 1667 1402 1560 1571 106.21 6.7% Sample 3 1656 1667 1402 1560 1571 106.21 6.7%
- Synonyms
- T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD80; CD80; CD28LG, CD28LG1, LAB7
- Gene Name
- CD80
- Gene ID
- 941
- UniProt
- P33681
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: V35-N242
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Monoclonal antibody from mouse
- Tissue Specificity
- Expressed on activated B-cells, macrophages and dendritic cells.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, B Cells, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 445 1656 163 425 1791 Standard deviation 7.29 34.71 69.55 7.98 34 100.29 CV (%) 4.5% 7.8% 4.2% 4.9% 8% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 445 1656 163 425 1791 Standard deviation 7.29 34.71 69.55 7.98 34 100.29 CV (%) 4.5% 7.8% 4.2% 4.9% 8% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 445 1656 163 425 1791 Mean (pg/ml) 162 445 1656 163 425 1791 Standard deviation 7.29 34.71 69.55 7.98 34 100.29 Standard deviation 7.29 34.71 69.55 7.98 34 100.29 CV (%) 4.5% 7.8% 4.2% 4.9% 8% 5.6% CV (%) 4.5% 7.8% 4.2% 4.9% 8% 5.6%
- Sensitivity
- <10 pg/mL
- Function
- Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 33048 MW
- Specificity
- Natural and recombinant human CD80
- Gene Name Synonym
- T-lymphocyte activation antigen CD80
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
- Protein Name
- T-lymphocyte activation antigen CD80
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.019 0.084 0.143 0.291 0.485 0.979 1.746 2.576 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.019 0.084 0.143 0.291 0.485 0.979 1.746 2.576 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.019 0.084 0.143 0.291 0.485 0.979 1.746 2.576 O.D. 0.019 0.084 0.143 0.291 0.485 0.979 1.746 2.576
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
