- Catalog number
- EK0709
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 156 159 178 161 9.54 5.9% Sample 2 448 401 380 439 417 27.7 6.6% Sample 3 1666 1734 1536 1629 1641 71.48 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 156 159 178 161 9.54 5.9% Sample 1 154 156 159 178 161 9.54 5.9% Sample 2 448 401 380 439 417 27.7 6.6% Sample 2 448 401 380 439 417 27.7 6.6% Sample 3 1666 1734 1536 1629 1641 71.48 4.3% Sample 3 1666 1734 1536 1629 1641 71.48 4.3%
- Synonyms
- B7.1 ; Cd80 ; B7.1
- Gene Name
- CD80
- Gene ID
- 25408
- UniProt
- O35187
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: I39-Q248
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, B Cells, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 448 1666 165 472 1688 Standard deviation 10.78 21.5 89.96 13.69 29.26 113.09 CV (%) 7% 4.8% 5.4% 8.3% 6.2% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 448 1666 165 472 1688 Standard deviation 10.78 21.5 89.96 13.69 29.26 113.09 CV (%) 7% 4.8% 5.4% 8.3% 6.2% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 154 448 1666 165 472 1688 Mean (pg/ml) 154 448 1666 165 472 1688 Standard deviation 10.78 21.5 89.96 13.69 29.26 113.09 Standard deviation 10.78 21.5 89.96 13.69 29.26 113.09 CV (%) 7% 4.8% 5.4% 8.3% 6.2% 6.7% CV (%) 7% 4.8% 5.4% 8.3% 6.2% 6.7%
- Sensitivity
- <2 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 36352 MW
- Specificity
- Natural and recombinant rat CD80
- Gene Name Synonym
- CD80 molecule
- Protein Name
- T-lymphocyte activation antigen CD80
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.092 0.162 0.316 0.576 1.062 1.525 2.013 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.092 0.162 0.316 0.576 1.062 1.525 2.013 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.092 0.162 0.316 0.576 1.062 1.525 2.013 O.D. 0.027 0.092 0.162 0.316 0.576 1.062 1.525 2.013
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
