- Catalog number
- EK0729
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3) . It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 31 30 30 30 0.7 2.3% Sample 2 155 156 165 162 159 4.15 2.6% Sample 3 411 409 455 402 419 20.9 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 31 30 30 30 0.7 2.3% Sample 1 29 31 30 30 30 0.7 2.3% Sample 2 155 156 165 162 159 4.15 2.6% Sample 2 155 156 165 162 159 4.15 2.6% Sample 3 411 409 455 402 419 20.9 4.9% Sample 3 411 409 455 402 419 20.9 4.9%
- Synonyms
- Platelet basic protein; PBP; C-X-C motif chemokine 7; Leukocyte-derived growth factor; LDGF; Macrophage-derived growth factor; MDGF; Small-inducible cytokine B7; Connective tissue-activating peptide III; CTAP-III; LA-PF4; Low-affinity platelet factor IV; TC-2; Connective tissue-activating peptide III (1-81) ; CTAP-III (1-81) ; Beta-thromboglobulin; Beta-TG; Neutrophil-activating peptide 2 (74) ; NAP-2 (74) ; Neutrophil-activating peptide 2 (73) ; NAP-2 (73) ; Neutrophil-activating peptide 2; NAP-2; TC-1; Neutrophil-activating peptide 2 (1-66) ; NAP-2 (1-66) ; Neutrophil-activating peptide 2 (1-63) ; NAP-2 (1-63) ; PPBP; CTAP3, CXCL7, SCYB7, TGB1, THBGB1
- Gene Name
- CXCL7
- Gene ID
- 5473
- UniProt
- P02775
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A59-D128
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cardiovascular, Chemokines, Immunology, Innate Immunity, Platelets
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 155 411 28 157 395 Standard deviation 1.47 7.9 29.18 1.73 9.26 32.78 CV (%) 8.3% 8.3% 7.1% 6.2% 5.9% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 155 411 28 157 395 Standard deviation 1.47 7.9 29.18 1.73 9.26 32.78 CV (%) 8.3% 8.3% 7.1% 6.2% 5.9% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 155 411 28 157 395 Mean (pg/ml) 29 155 411 28 157 395 Standard deviation 1.47 7.9 29.18 1.73 9.26 32.78 Standard deviation 1.47 7.9 29.18 1.73 9.26 32.78 CV (%) 8.3% 8.3% 7.1% 6.2% 5.9% 8.3% CV (%) 8.3% 8.3% 7.1% 6.2% 5.9% 8.3%
- Sensitivity
- <2 pg/mL
- Function
- LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2 (73), NAP-2 (74), NAP-2 (1-66), and most potent NAP-2 (1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III (1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 13894 MW
- Specificity
- Natural and recombinant human CXCL7
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Platelet basic protein
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Protein Name
- Platelet basic protein
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.015 0.055 0.096 0.177 0.346 0.704 1.343 1.882 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.015 0.055 0.096 0.177 0.346 0.704 1.343 1.882 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.015 0.055 0.096 0.177 0.346 0.704 1.343 1.882 O.D. 0.015 0.055 0.096 0.177 0.346 0.704 1.343 1.882
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
