- Catalog number
- EK0735
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10) . CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 54 60 64 59 3.57 6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 3 879 923 848 978 907 48.89 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 54 60 64 59 3.57 6% Sample 1 60 54 60 64 59 3.57 6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 3 879 923 848 978 907 48.89 5.3% Sample 3 879 923 848 978 907 48.89 5.3%
- Synonyms
- C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein; Gamma-IP10; IP-10; Small-inducible cytokine B10; CXCL10 (1-73) ; CXCL10; INP10, SCYB10
- Gene Name
- CXCL10
- Gene ID
- 3627
- UniProt
- P02778
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: V22-P98
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Chemokines, Immunology, Innate Immunity, Invasion/Microenvironment
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5%
- Sensitivity
- <1 pg/mL
- Function
- Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 10881 MW
- Specificity
- Natural and recombinant human CXCL10
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-X-C motif chemokine 10
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Protein Name
- C-X-C motif chemokine 10
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.136 0.206 0.345 0.535 0.998 1.625 2.684 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.136 0.206 0.345 0.535 0.998 1.625 2.684 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.136 0.206 0.345 0.535 0.998 1.625 2.684 O.D. 0.038 0.136 0.206 0.345 0.535 0.998 1.625 2.684
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
