Mouse CXCL10/IP-10 ELISA Kit PicoKine®

About this product

Mouse CXCL10/IP-10 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Cxcl10 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0736

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10) . CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 98 104 102 102 2.68 2.6% Sample 2 245 274 275 259 263 12.29 4.6% Sample 3 923 908 845 862 884 32.01 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 98 104 102 102 2.68 2.6% Sample 1 105 98 104 102 102 2.68 2.6% Sample 2 245 274 275 259 263 12.29 4.6% Sample 2 245 274 275 259 263 12.29 4.6% Sample 3 923 908 845 862 884 32.01 3.6% Sample 3 923 908 845 862 884 32.01 3.6%

Synonyms

C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein; Gamma-IP10; IP-10; C7; Interferon-gamma induced protein CRG-2; Small-inducible cytokine B10; Cxcl10; Crg2, Ifi10, Inp10, Scyb10

Gene Name

CXCL10

Gene ID

15945

UniProt

P17515

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I22-P98

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in the spleen, thymus, lymph nodes and liver. .

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Chemokines, Immunology, Innate Immunity, Invasion/Microenvironment

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 245 923 110 248 879 Standard deviation 10.237 13.72 47.99 10.23 18.6 56.25 CV (%) 7.5% 5.6% 5.2% 9.3% 7.5% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 245 923 110 248 879 Standard deviation 10.237 13.72 47.99 10.23 18.6 56.25 CV (%) 7.5% 5.6% 5.2% 9.3% 7.5% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 105 245 923 110 248 879 Mean (pg/ml) 105 245 923 110 248 879 Standard deviation 10.237 13.72 47.99 10.23 18.6 56.25 Standard deviation 10.237 13.72 47.99 10.23 18.6 56.25 CV (%) 7.5% 5.6% 5.2% 9.3% 7.5% 6.4% CV (%) 7.5% 5.6% 5.2% 9.3% 7.5% 6.4%

Sensitivity

<1 pg/mL

Function

In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10789 MW

Specificity

Natural and recombinant mouse CXCL10

Anticoagulant

Heparin

Gene Name Synonym

C-X-C motif chemokine 10

Subcellular Location

Secreted.

Protein Name

C-X-C motif chemokine 10

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.062 0.088 0.163 0.286 0.516 1.002 1.854 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.062 0.088 0.163 0.286 0.516 1.002 1.854 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.062 0.088 0.163 0.286 0.516 1.002 1.854 O.D. 0.024 0.062 0.088 0.163 0.286 0.516 1.002 1.854

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.