- Catalog number
- EK0737
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant (I-TAC) and Interferon-gamma-inducible protein 9 (IP-9) . By PCR analysis of somatic cell hybrids, the CXCL11 gene was mapped to chromosome 4, and using FISH, it was located to 4q21.2. The CXCL11 was strongly induced by IFNG and beta-interferon, but only weakly induced by alpha-interferon. Induction of CXCL11 required expression of STAT1. What's more, it found that monocyte chemotaxis occurs in response to recombinant CXCL11. RT-PCR analysis detected CXCL11 expression in brain tissue from a patient with AIDS dementia and a patient with multiple sclerosis but not in 2 controls.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 107 122 102 116 111 7.75 6.9% Sample 2 712 667 661 672 678 20.01 2.9% Sample 3 1603 1507 1537 1543 1547 34.82 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 107 122 102 116 111 7.75 6.9% Sample 1 107 122 102 116 111 7.75 6.9% Sample 2 712 667 661 672 678 20.01 2.9% Sample 2 712 667 661 672 678 20.01 2.9% Sample 3 1603 1507 1537 1543 1547 34.82 2.2% Sample 3 1603 1507 1537 1543 1547 34.82 2.2%
- Synonyms
- C-X-C motif chemokine 11; Beta-R1; H174; Interferon gamma-inducible protein 9; IP-9; Interferon-inducible T-cell alpha chemoattractant; I-TAC; Small-inducible cytokine B11; CXCL11; ITAC, SCYB11, SCYB9B
- Gene Name
- CXCL11
- Gene ID
- 6373
- UniProt
- O14625
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: F22-F94
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
- Applications
- ELISA
- Field of Research
- Autoimmune, Chemokines, Immune System Diseases, Immunology, Innate Immunity, Neurodegenerative Disease, Neurology Process, Neuroscience
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 107 712 1603 100 764 1565 Standard deviation 7.91 47.7 83.35 8.7 64.94 82.94 CV (%) 7.4% 6.7% 5.2% 8.7% 8.5% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 107 712 1603 100 764 1565 Standard deviation 7.91 47.7 83.35 8.7 64.94 82.94 CV (%) 7.4% 6.7% 5.2% 8.7% 8.5% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 107 712 1603 100 764 1565 Mean (pg/ml) 107 712 1603 100 764 1565 Standard deviation 7.91 47.7 83.35 8.7 64.94 82.94 Standard deviation 7.91 47.7 83.35 8.7 64.94 82.94 CV (%) 7.4% 6.7% 5.2% 8.7% 8.5% 5.3% CV (%) 7.4% 6.7% 5.2% 8.7% 8.5% 5.3%
- Sensitivity
- <10 pg/mL
- Function
- Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 10365 MW
- Specificity
- Natural and recombinant human CXCL11
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-X-C motif chemokine 11
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Protein Name
- C-X-C motif chemokine 11
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.053 0.096 0.178 0.352 0.681 1.249 2.342 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.053 0.096 0.178 0.352 0.681 1.249 2.342 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.053 0.096 0.178 0.352 0.681 1.249 2.342 O.D. 0.013 0.053 0.096 0.178 0.352 0.681 1.249 2.342
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
