- Catalog number
- EK0740
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 34 32 29 31 1.8 5.8% Sample 2 182 204 199 194 194 8.16 4.2% Sample 3 491 507 522 441 490 30.47 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 34 32 29 31 1.8 5.8% Sample 1 31 34 32 29 31 1.8 5.8% Sample 2 182 204 199 194 194 8.16 4.2% Sample 2 182 204 199 194 194 8.16 4.2% Sample 3 491 507 522 441 490 30.47 6.2% Sample 3 491 507 522 441 490 30.47 6.2%
- Synonyms
- C-X-C motif chemokine 13; B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13; Cxcl13; Blc, Scyb13
- Gene Name
- CXCL13
- Gene ID
- 55985
- UniProt
- O55038
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: I22-A109
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles) .
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5%
- Sensitivity
- <10 pg/mL
- Function
- Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in ing the migration of B-lymphocytes to follicles in secondary lymphoid organs.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 11927 MW
- Specificity
- Natural and recombinant mouse CXCL13
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-X-C motif chemokine 13
- Subcellular Location
- Secreted.
- Protein Name
- C-X-C motif chemokine 13
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.031 0.102 0.178 0.329 0.708 1.163 1.851 2.431 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.031 0.102 0.178 0.329 0.708 1.163 1.851 2.431 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.031 0.102 0.178 0.329 0.708 1.163 1.851 2.431 O.D. 0.031 0.102 0.178 0.329 0.708 1.163 1.851 2.431
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
