- Catalog number
- EK0741
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16 (CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 112 116 125 118 5.06 4.2% Sample 2 725 748 679 765 729 32.29 4.4% Sample 3 2351 2384 2122 2446 2325 122.47 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 112 116 125 118 5.06 4.2% Sample 1 122 112 116 125 118 5.06 4.2% Sample 2 725 748 679 765 729 32.29 4.4% Sample 2 725 748 679 765 729 32.29 4.4% Sample 3 2351 2384 2122 2446 2325 122.47 5.2% Sample 3 2351 2384 2122 2446 2325 122.47 5.2%
- Synonyms
- C-X-C motif chemokine 16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SR-PSOX; Small-inducible cytokine B16; Transmembrane chemokine CXCL16; CXCL16; SCYB16, SRPSOX; UNQ2759/PRO6714
- Gene Name
- CXCL16
- Gene ID
- 58191
- UniProt
- Q9H2A7
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: N49-P137
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 93.7 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 725 2351 133 696 2150 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 725 2351 133 696 2150 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 122 725 2351 133 696 2150 Mean (pg/ml) 122 725 2351 133 696 2150 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2% CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2%
- Sensitivity
- <10 pg/mL
- Function
- Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity) . Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 27579 MW
- Specificity
- Natural and recombinant human CXCL16
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-X-C motif chemokine 16
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein . Secreted. Also exists as a soluble form.
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Protein Name
- C-X-C motif chemokine 16
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.011 0.103 0.168 0.312 0.609 0.933 1.546 2.068 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.011 0.103 0.168 0.312 0.609 0.933 1.546 2.068 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.011 0.103 0.168 0.312 0.609 0.933 1.546 2.068 O.D. 0.011 0.103 0.168 0.312 0.609 0.933 1.546 2.068
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
