- Catalog number
- EK0752
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2) .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 83 83 80 84 3.67 4.3% Sample 2 269 235 269 222 248 20.76 8.3% Sample 3 832 815 926 825 849 44.57 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 83 83 80 84 3.67 4.3% Sample 1 90 83 83 80 84 3.67 4.3% Sample 2 269 235 269 222 248 20.76 8.3% Sample 2 269 235 269 222 248 20.76 8.3% Sample 3 832 815 926 825 849 44.57 5.2% Sample 3 832 815 926 825 849 44.57 5.2%
- Synonyms
- Endothelial cell-specific molecule 1; ESM-1; ESM1
- Gene Name
- ESM1
- Gene ID
- 11082
- UniProt
- Q9NQ30
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: W20-R184
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.
- Applications
- ELISA
- Field of Research
- Cell Type Markers, Tags & Cell Markers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 269 832 96 293 860 Standard deviation 7.11 14.79 45.76 8.44 19.33 54.18 CV (%) 7.9% 5.5% 5.5% 8.8% 6.6% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 269 832 96 293 860 Standard deviation 7.11 14.79 45.76 8.44 19.33 54.18 CV (%) 7.9% 5.5% 5.5% 8.8% 6.6% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 269 832 96 293 860 Mean (pg/ml) 90 269 832 96 293 860 Standard deviation 7.11 14.79 45.76 8.44 19.33 54.18 Standard deviation 7.11 14.79 45.76 8.44 19.33 54.18 CV (%) 7.9% 5.5% 5.5% 8.8% 6.6% 6.3% CV (%) 7.9% 5.5% 5.5% 8.8% 6.6% 6.3%
- Sensitivity
- <10 pg/mL
- Function
- Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 20095 MW
- Specificity
- Natural and recombinant human ESM1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Endothelial cell-specific molecule 1
- Subcellular Location
- Secreted.
- Protein Name
- Endothelial cell-specific molecule 1
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.079 0.117 0.196 0.335 0.596 1.061 1.836 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.079 0.117 0.196 0.335 0.596 1.061 1.836 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.079 0.117 0.196 0.335 0.596 1.061 1.836 O.D. 0.027 0.079 0.117 0.196 0.335 0.596 1.061 1.836
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
