Human Fetuin A/AHSG ELISA Kit PicoKine®

About this product

Human Fetuin A / AHSG ELISA Kit PicoKine® (96 Tests) . Quantitate Human AHSG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0757

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1646 1726 1766 1528 1666 90.88 5.4% Sample 2 4628 4495 4603 4388 4528 95.27 2.1% Sample 3 22095 25102 22613 25109 23729 1387.88 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1646 1726 1766 1528 1666 90.88 5.4% Sample 1 1646 1726 1766 1528 1666 90.88 5.4% Sample 2 4628 4495 4603 4388 4528 95.27 2.1% Sample 2 4628 4495 4603 4388 4528 95.27 2.1% Sample 3 22095 25102 22613 25109 23729 1387.88 5.8% Sample 3 22095 25102 22613 25109 23729 1387.88 5.8%

Synonyms

Alpha-2-HS-glycoprotein; Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; Fetuin-A; Alpha-2-HS-glycoprotein chain A; Alpha-2-HS-glycoprotein chain B; AHSG; FETUA; PRO2743

Gene Name

AHSG

Gene ID

197

UniProt

P02765

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A19-V367

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.

Applications

ELISA

Field of Research

Cardiovascular, Metabolism, Neurogenesis, Neurology Process, Neuroscience, Obesity, Serum Proteins

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1646 4628 22095 1708 5021 21195 Standard deviation 126.74 185.82 883.8 143.47 2244 1229.31 CV (%) 7.7% 4% 4% 8.4% 4.5% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1646 4628 22095 1708 5021 21195 Standard deviation 126.74 185.82 883.8 143.47 2244 1229.31 CV (%) 7.7% 4% 4% 8.4% 4.5% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1646 4628 22095 1708 5021 21195 Mean (pg/ml) 1646 4628 22095 1708 5021 21195 Standard deviation 126.74 185.82 883.8 143.47 2244 1229.31 Standard deviation 126.74 185.82 883.8 143.47 2244 1229.31 CV (%) 7.7% 4% 4% 8.4% 4.5% 5.8% CV (%) 7.7% 4% 4% 8.4% 4.5% 5.8%

Sensitivity

<10 pg/mL

Function

Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

39325 MW

Specificity

Natural and recombinant human Fetuin A

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Alpha-2-HS-glycoprotein

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the fetuin family.

Protein Name

Alpha-2-HS-glycoprotein

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.001 0.041 0.09 0.169 0.336 0.68 1.15 1.821 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.001 0.041 0.09 0.169 0.336 0.68 1.15 1.821 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.001 0.041 0.09 0.169 0.336 0.68 1.15 1.821 O.D. 0.001 0.041 0.09 0.169 0.336 0.68 1.15 1.821

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.