- Catalog number
- EK0763
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse. They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 316 342 346 336 12.07 3.5% Sample 2 1747 1764 1871 1625 1751 87.26 4.9% Sample 3 4774 4662 4642 4948 4756 121.46 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 316 342 346 336 12.07 3.5% Sample 1 343 316 342 346 336 12.07 3.5% Sample 2 1747 1764 1871 1625 1751 87.26 4.9% Sample 2 1747 1764 1871 1625 1751 87.26 4.9% Sample 3 4774 4662 4642 4948 4756 121.46 2.5% Sample 3 4774 4662 4642 4948 4756 121.46 2.5%
- Synonyms
- Galectin-1; Gal-1;14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1; Lgals1; Gbp
- Gene Name
- LGALS1
- Gene ID
- 16852
- UniProt
- P16045
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A2-E135
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cell Biology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 1747 4774 318 1697 4684 Standard deviation 22.29 132.77 300.76 20.67 144.24 304.46 CV (%) 6.5% 7.6% 8.5% 6.5% 8.5% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 1747 4774 318 1697 4684 Standard deviation 22.29 132.77 300.76 20.67 144.24 304.46 CV (%) 6.5% 7.6% 8.5% 6.5% 8.5% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 343 1747 4774 318 1697 4684 Mean (pg/ml) 343 1747 4774 318 1697 4684 Standard deviation 22.29 132.77 300.76 20.67 144.24 304.46 Standard deviation 22.29 132.77 300.76 20.67 144.24 304.46 CV (%) 6.5% 7.6% 8.5% 6.5% 8.5% 6.5% CV (%) 6.5% 7.6% 8.5% 6.5% 8.5% 6.5%
- Sensitivity
- <5 pg/mL
- Function
- May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity. Strong inducer of T-cell apoptosis (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 14866 MW
- Specificity
- Natural and recombinant mouse Galectin-1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Galectin-1
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Sequence Similarities
- Contains 1 galectin domain.
- Protein Name
- Galectin-1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
