- Catalog number
- EK0764
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 212 196 200 216 206 8.24 4% Sample 2 1876 2001 1983 1897 1939 53.64 2.7% Sample 3 4230 4475 3962 4238 4226 181.56 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 212 196 200 216 206 8.24 4% Sample 1 212 196 200 216 206 8.24 4% Sample 2 1876 2001 1983 1897 1939 53.64 2.7% Sample 2 1876 2001 1983 1897 1939 53.64 2.7% Sample 3 4230 4475 3962 4238 4226 181.56 4.2% Sample 3 4230 4475 3962 4238 4226 181.56 4.2%
- Synonyms
- Galectin-3; Gal-3;35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3; MAC2
- Gene Name
- LGALS3
- Gene ID
- 3958
- UniProt
- P17931
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A2-I250
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. .
- Applications
- ELISA
- Field of Research
- Cancer, Cell Cycle, Cell Division, Oncoproteins, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 212 1876 4230 205 1951 4307 Standard deviation 16.74 76.91 334.17 19.88 118.70 374.7 CV (%) 7.9% 4.1% 7.9% 9.7% 5.9% 8.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 212 1876 4230 205 1951 4307 Standard deviation 16.74 76.91 334.17 19.88 118.70 374.7 CV (%) 7.9% 4.1% 7.9% 9.7% 5.9% 8.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 212 1876 4230 205 1951 4307 Mean (pg/ml) 212 1876 4230 205 1951 4307 Standard deviation 16.74 76.91 334.17 19.88 118.70 374.7 Standard deviation 16.74 76.91 334.17 19.88 118.70 374.7 CV (%) 7.9% 4.1% 7.9% 9.7% 5.9% 8.7% CV (%) 7.9% 4.1% 7.9% 9.7% 5.9% 8.7%
- Sensitivity
- <10 pg/mL
- Function
- Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity) . In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 26152 MW
- Specificity
- Natural and recombinant human Galectin-3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Galectin-3
- Subcellular Location
- Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface.
- Sequence Similarities
- Contains 1 galectin domain.
- Protein Name
- Galectin-3
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.107 0.206 0.358 0.701 1.281 1.83 2.092 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.107 0.206 0.358 0.701 1.281 1.83 2.092 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.107 0.206 0.358 0.701 1.281 1.83 2.092 O.D. 0.001 0.107 0.206 0.358 0.701 1.281 1.83 2.092
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
