- Catalog number
- EK0765
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 77 88 80 81 4.03 4.9% Sample 2 434 488 515 469 476 29.48 6.1% Sample 3 1619 1792 1773 1612 1699 83.8 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 77 88 80 81 4.03 4.9% Sample 1 81 77 88 80 81 4.03 4.9% Sample 2 434 488 515 469 476 29.48 6.1% Sample 2 434 488 515 469 476 29.48 6.1% Sample 3 1619 1792 1773 1612 1699 83.8 4.9% Sample 3 1619 1792 1773 1612 1699 83.8 4.9%
- Synonyms
- Galectin ; Lgals3 ; mCG_6908
- Gene Name
- LGALS3
- Gene ID
- 16854
- UniProt
- Q8C253
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A2-I264
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cell Cycle, Cell Division, Oncoproteins, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 434 1619 80 402 1701 Standard deviation 8.1%1 31.24 126.28 7.68 32.56 153.09 CV (%) 7.8% 7.2% 7.8% 9.6% 8.1% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 434 1619 80 402 1701 Standard deviation 8.1%1 31.24 126.28 7.68 32.56 153.09 CV (%) 7.8% 7.2% 7.8% 9.6% 8.1% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 81 434 1619 80 402 1701 Mean (pg/ml) 81 434 1619 80 402 1701 Standard deviation 8.1%1 31.24 126.28 7.68 32.56 153.09 Standard deviation 8.1%1 31.24 126.28 7.68 32.56 153.09 CV (%) 7.8% 7.2% 7.8% 9.6% 8.1% 9% CV (%) 7.8% 7.2% 7.8% 9.6% 8.1% 9%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 27415 MW
- Specificity
- Natural and recombinant mouse Galectin-3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Galectin
- Protein Name
- Galectin-3
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
