- Catalog number
- EK0777
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- IL6R alpha (IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 48 54 48 51 3 5.8% Sample 2 271 254 262 234 255 13.66 5.3% Sample 3 821 928 965 877 897 54.21 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 48 54 48 51 3 5.8% Sample 1 54 48 54 48 51 3 5.8% Sample 2 271 254 262 234 255 13.66 5.3% Sample 2 271 254 262 234 255 13.66 5.3% Sample 3 821 928 965 877 897 54.21 6% Sample 3 821 928 965 877 897 54.21 6%
- Synonyms
- Interleukin 6 receptor ; Interleukin 6 receptor, isoform CRA_a ; IL6R ; hCG_1810911
- Gene Name
- IL6R
- Gene ID
- 3570
- UniProt
- A0N0L5
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L20-D358
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Interleukins, Lymphoid, Myeloid, Stem Cells, T Lymphocytic Lineage
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 271 821 59 259 773 Standard deviation 2.86 17.07 41.87 3.77 17.35 44.06 CV (%) 5.3% 6.7% 5.7% 6.4% 6.7% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 271 821 59 259 773 Standard deviation 2.86 17.07 41.87 3.77 17.35 44.06 CV (%) 5.3% 6.7% 5.7% 6.4% 6.7% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 54 271 821 59 259 773 Mean (pg/ml) 54 271 821 59 259 773 Standard deviation 2.86 17.07 41.87 3.77 17.35 44.06 Standard deviation 2.86 17.07 41.87 3.77 17.35 44.06 CV (%) 5.3% 6.7% 5.7% 6.4% 6.7% 5.7% CV (%) 5.3% 6.7% 5.7% 6.4% 6.7% 5.7%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 51548 MW
- Specificity
- Natural and recombinant human IL-6R alpha
- Anticoagulant
- Heparin, EDTA or Citrate
- Gene Name Synonym
- Interleukin 6 receptor
- Protein Name
- Interleukin-6 receptor subunit alpha
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.127 0.21 0.356 0.613 1.012 1.615 2.39 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.127 0.21 0.356 0.613 1.012 1.615 2.39 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.127 0.21 0.356 0.613 1.012 1.615 2.39 O.D. 0.031 0.127 0.21 0.356 0.613 1.012 1.615 2.39
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
