- Catalog number
- EK0797
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T (H) 17 cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 176 199 176 180 182 9.52 5.2% Sample 2 862 737 773 850 805 52.25 6.4% Sample 3 1322 1296 1226 1149 1248 67.2 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 176 199 176 180 182 9.52 5.2% Sample 1 176 199 176 180 182 9.52 5.2% Sample 2 862 737 773 850 805 52.25 6.4% Sample 2 862 737 773 850 805 52.25 6.4% Sample 3 1322 1296 1226 1149 1248 67.2 5.3% Sample 3 1322 1296 1226 1149 1248 67.2 5.3%
- Synonyms
- Interleukin ; Il21 ; mCG_12674
- Gene Name
- IL21
- Gene ID
- 60505
- UniProt
- Q9ES17
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: P25-S146
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 176 862 1322 188 946 1262 Standard deviation 9.32 43.96 96.50 9.96 52.97 107.27 CV (%) 5.3% 8.5% 7.3% 5.3% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 176 862 1322 188 946 1262 Standard deviation 9.32 43.96 96.50 9.96 52.97 107.27 CV (%) 5.3% 8.5% 7.3% 5.3% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 176 862 1322 188 946 1262 Mean (pg/ml) 176 862 1322 188 946 1262 Standard deviation 9.32 43.96 96.50 9.96 52.97 107.27 Standard deviation 9.32 43.96 96.50 9.96 52.97 107.27 CV (%) 5.3% 8.5% 7.3% 5.3% 5.6% 8.5% CV (%) 5.3% 8.5% 7.3% 5.3% 5.6% 8.5%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 16811 MW
- Specificity
- Natural and recombinant mouse IL-21
- Gene Name Synonym
- Interleukin
- Protein Name
- Interleukin-21
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.042 0.08 0.14 0.246 0.46 0.894 1.869 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.042 0.08 0.14 0.246 0.46 0.894 1.869 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.042 0.08 0.14 0.246 0.46 0.894 1.869 O.D. 0.008 0.042 0.08 0.14 0.246 0.46 0.894 1.869
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
