- Catalog number
- EK0814
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Macrophage Stimulating 1 (SMT1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21) . The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 250 301 271 291 278 19.56 7% Sample 2 1105 1177 1061 1161 1126 46.07 4% Sample 3 6318 6544 5798 6359 6254 277.1 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 250 301 271 291 278 19.56 7% Sample 1 250 301 271 291 278 19.56 7% Sample 2 1105 1177 1061 1161 1126 46.07 4% Sample 2 1105 1177 1061 1161 1126 46.07 4% Sample 3 6318 6544 5798 6359 6254 277.1 4.4% Sample 3 6318 6544 5798 6359 6254 277.1 4.4%
- Synonyms
- Hepatocyte growth factor-like protein; Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; Hepatocyte growth factor-like protein alpha chain; Hepatocyte growth factor-like protein beta chain; MST1; D3F15S2, DNF15S2, HGFL
- Gene Name
- MST1
- Gene ID
- 4485
- UniProt
- P26927
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q19-G711
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Monoclonal antibody from mouse
- Applications
- ELISA
- Field of Research
- Cancer, Immunology, Innate Immunity, Macrophage/Inflammation, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 250 1105 6318 245 1017 6055 Standard deviation 19.25 44.2 398.03 23.52 53.9 399.63 CV (%) 7.7% 4% 5.3%% 9.6% 5.3% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 250 1105 6318 245 1017 6055 Standard deviation 19.25 44.2 398.03 23.52 53.9 399.63 CV (%) 7.7% 4% 5.3%% 9.6% 5.3% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 250 1105 6318 245 1017 6055 Mean (pg/ml) 250 1105 6318 245 1017 6055 Standard deviation 19.25 44.2 398.03 23.52 53.9 399.63 Standard deviation 19.25 44.2 398.03 23.52 53.9 399.63 CV (%) 7.7% 4% 5.3%% 9.6% 5.3% 6.6% CV (%) 7.7% 4% 5.3%% 9.6% 5.3% 6.6%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 80320 MW
- Specificity
- Natural and recombinant human MSP
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Hepatocyte growth factor-like protein
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the peptidase S1 family. Plasminogen subfamily.
- Protein Name
- Hepatocyte growth factor-like protein
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.121 0.183 0.317 0.543 0.968 1.566 2.037 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.121 0.183 0.317 0.543 0.968 1.566 2.037 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.121 0.183 0.317 0.543 0.968 1.566 2.037 O.D. 0.036 0.121 0.183 0.317 0.543 0.968 1.566 2.037
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
