Human Prostate-specific antigen ELISA kit (PSA/KLK3) PicoKine®

About this product

Human Kallikrein 3/PSA/KLK3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human KLK3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0816

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Prostate-specific antigen (PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome (19q13) .3

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 551 505 496 595 536 39.57 7.3% Sample 2 2528 2531 2745 2357 2540 137.6 5.4% Sample 3 8207 9826 9283 9589 9226 619.14 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 551 505 496 595 536 39.57 7.3% Sample 1 551 505 496 595 536 39.57 7.3% Sample 2 2528 2531 2745 2357 2540 137.6 5.4% Sample 2 2528 2531 2745 2357 2540 137.6 5.4% Sample 3 8207 9826 9283 9589 9226 619.14 6.7% Sample 3 8207 9826 9283 9589 9226 619.14 6.7%

Synonyms

Prostate-specific antigen; PSA;3.4.21.77; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase; KLK3; APS

Gene Name

KLK3

Gene ID

354

UniProt

P07288

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A18-P261

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Type Markers, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Neuroscience, Signal Transduction, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 551 2528 8207 568 2328 8686 Standard deviation 36.91 156.73 648.35 47.14 144.33 773.05 CV (%) 6.7% 6.2% 7.9% 8.3% 6.2% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 551 2528 8207 568 2328 8686 Standard deviation 36.91 156.73 648.35 47.14 144.33 773.05 CV (%) 6.7% 6.2% 7.9% 8.3% 6.2% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 551 2528 8207 568 2328 8686 Mean (pg/ml) 551 2528 8207 568 2328 8686 Standard deviation 36.91 156.73 648.35 47.14 144.33 773.05 Standard deviation 36.91 156.73 648.35 47.14 144.33 773.05 CV (%) 6.7% 6.2% 7.9% 8.3% 6.2% 8.9% CV (%) 6.7% 6.2% 7.9% 8.3% 6.2% 8.9%

Sensitivity

<10 pg/mL

Function

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

28741 MW

Specificity

Natural and recombinant human Kallikrein 3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Prostate-specific antigen

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein Name

Prostate-specific antigen

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.056 0.128 0.176 0.261 0.411 0.7 1.199 1.791 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.056 0.128 0.176 0.261 0.411 0.7 1.199 1.791 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.056 0.128 0.176 0.261 0.411 0.7 1.199 1.791 O.D. 0.056 0.128 0.176 0.261 0.411 0.7 1.199 1.791

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.