- Catalog number
- EK0817
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 128 140 148 137 7.39 5.3% Sample 2 809 798 776 808 797 13.27 1.6% Sample 3 2161 2628 2234 2197 2305 188.26 8.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 128 140 148 137 7.39 5.3% Sample 1 134 128 140 148 137 7.39 5.3% Sample 2 809 798 776 808 797 13.27 1.6% Sample 2 809 798 776 808 797 13.27 1.6% Sample 3 2161 2628 2234 2197 2305 188.26 8.1% Sample 3 2161 2628 2234 2197 2305 188.26 8.1%
- Synonyms
- Kallikrein-5;3.4.21.-; Kallikrein-like protein 2; KLK-L2; Stratum corneum tryptic enzyme; KLK5; SCTE; UNQ570/PRO1132
- Gene Name
- KLK5
- Gene ID
- 25818
- UniProt
- Q9Y337
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: V23-S293
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin. .
- Applications
- ELISA
- Field of Research
- Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva.
- Detection Range
- 78 pg/mL - 5,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 809 2161 146 747 2368 Standard deviation 5.36 33.97 133.98 8.32 32.12 149.18 CV (%) 4% 4.2% 6.2% 5.7% 4.3% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 809 2161 146 747 2368 Standard deviation 5.36 33.97 133.98 8.32 32.12 149.18 CV (%) 4% 4.2% 6.2% 5.7% 4.3% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 134 809 2161 146 747 2368 Mean (pg/ml) 134 809 2161 146 747 2368 Standard deviation 5.36 33.97 133.98 8.32 32.12 149.18 Standard deviation 5.36 33.97 133.98 8.32 32.12 149.18 CV (%) 4% 4.2% 6.2% 5.7% 4.3% 6.3% CV (%) 4% 4.2% 6.2% 5.7% 4.3% 6.3%
- Sensitivity
- <10 pg/mL
- Function
- May be involved in desquamation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 32019 MW
- Specificity
- Natural and recombinant human KLK5
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Kallikrein-5
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the peptidase S1 family. Kallikrein subfamily.
- Protein Name
- Kallikrein-5
- Sample Data
- Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.065 0.105 0.175 0.311 0.657 1.054 1.877 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.065 0.105 0.175 0.311 0.657 1.054 1.877 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.065 0.105 0.175 0.311 0.657 1.054 1.877 O.D. 0.025 0.065 0.105 0.175 0.311 0.657 1.054 1.877
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
