- Catalog number
- EK0820
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 86 90 98 93 5.72 6.1% Sample 2 261 290 276 276 275 10.25 3.7% Sample 3 837 918 880 908 885 31.4 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 86 90 98 93 5.72 6.1% Sample 1 100 86 90 98 93 5.72 6.1% Sample 2 261 290 276 276 275 10.25 3.7% Sample 2 261 290 276 276 275 10.25 3.7% Sample 3 837 918 880 908 885 31.4 3.5% Sample 3 837 918 880 908 885 31.4 3.5%
- Synonyms
- Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF
- Gene Name
- FGF7
- Gene ID
- 2252
- UniProt
- P21781
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: C32-T194
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Epithelial cell.
- Applications
- ELISA
- Field of Research
- Cancer, Developmental Biology, Growth Factors, Growth Factors/Hormones, Organogenesis, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 261 837 97 261 764 Standard deviation 7.5 16.18 41.85 7.66 20.35 45.84 CV (%) 7.5% 6.2% 5% 7.9% 7.8% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 261 837 97 261 764 Standard deviation 7.5 16.18 41.85 7.66 20.35 45.84 CV (%) 7.5% 6.2% 5% 7.9% 7.8% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 100 261 837 97 261 764 Mean (pg/ml) 100 261 837 97 261 764 Standard deviation 7.5 16.18 41.85 7.66 20.35 45.84 Standard deviation 7.5 16.18 41.85 7.66 20.35 45.84 CV (%) 7.5% 6.2% 5% 7.9% 7.8% 6% CV (%) 7.5% 6.2% 5% 7.9% 7.8% 6%
- Sensitivity
- <4 pg/mL
- Function
- Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 22509 MW
- Specificity
- Natural and recombinant human FGF7
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Fibroblast growth factor 7
- Subcellular Location
- Secreted.
- Protein Name
- Fibroblast growth factor 7
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.089 0.179 0.331 0.576 0.991 1.486 1.991 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.089 0.179 0.331 0.576 0.991 1.486 1.991 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.089 0.179 0.331 0.576 0.991 1.486 1.991 O.D. 0.009 0.089 0.179 0.331 0.576 0.991 1.486 1.991
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
