Mouse LOX-1/OLR1 ELISA Kit PicoKine®

About this product

Mouse LOX-1/OLR1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Olr1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0825

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a protein that in humans is encoded by the OLR1 gene. It belongs to the C-type lectin superfamily. The LOX1 gene is mapped to to 12p13-p12. The protein has got the amino acids of 363. It can be detected in various tissues, highly expressed in placenta. This protein may be involved in the regulation of Fas-induced apoptosis and play a role as a scavenger receptor. The standards of this kit are recombinant mouse LOX-1 (R60-I363), with molecular weight of 36.3kDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 102 95 104 99 4.06 4.1% Sample 2 321 304 284 296 301 13.44 4.4% Sample 3 767 794 819 788 792 18.53 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 102 95 104 99 4.06 4.1% Sample 1 95 102 95 104 99 4.06 4.1% Sample 2 321 304 284 296 301 13.44 4.4% Sample 2 321 304 284 296 301 13.44 4.4% Sample 3 767 794 819 788 792 18.53 2.3% Sample 3 767 794 819 788 792 18.53 2.3%

Synonyms

Oxidized low-density lipoprotein receptor 1; Ox-LDL receptor 1; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; Oxidized low-density lipoprotein receptor 1, soluble form; Olr1; Lox1

Gene Name

OLR1

Gene ID

108078

UniProt

Q9EQ09

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R60-I363

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Cell Biology, Fatty Acids, Lipid Metabolism, Lipids/Lipoproteins, Metabolism, Oxidative Stress, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 321 767 85 312 750 Standard deviation 4.84 17.33 60.59 5.35 22.46 61.5 CV (%) 8.2% 5.4% 7.9% 7.2% 7.2% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 321 767 85 312 750 Standard deviation 4.84 17.33 60.59 5.35 22.46 61.5 CV (%) 8.2% 5.4% 7.9% 7.2% 7.2% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 85 321 767 85 312 750 Mean (pg/ml) 85 321 767 85 312 750 Standard deviation 4.84 17.33 60.59 5.35 22.46 61.5 Standard deviation 4.84 17.33 60.59 5.35 22.46 61.5 CV (%) 8.2% 5.4% 7.9% 7.2% 7.2% 8.2% CV (%) 8.2% 5.4% 7.9% 7.2% 7.2% 8.2%

Sensitivity

<1 pg/mL

Function

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria (By similarity) . .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

41643 MW

Specificity

Natural and recombinant mouse LOX-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Oxidized low-density lipoprotein receptor 1

Subcellular Location

Cell membrane ; Lipid-anchor . Cell membrane ; Single-pass type II membrane protein . Membrane raft . Secreted . A secreted form also exists. Localization to membrane rafts requires palmitoylation (By similarity) . .

Sequence Similarities

Contains 1 C-type lectin domain.

Protein Name

Oxidized low-density lipoprotein receptor 1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.114 0.186 0.333 0.547 0.988 1.543 2.559 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.114 0.186 0.333 0.547 0.988 1.543 2.559 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.114 0.186 0.333 0.547 0.988 1.543 2.559 O.D. 0.002 0.114 0.186 0.333 0.547 0.988 1.543 2.559

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.