- Catalog number
- EK0842
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 115 126 117 122 120 4.3 3.5% Sample 2 865 746 848 804 815 46.01 5.6% Sample 3 2242 2225 2256 2223 2236 13.46 0.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 115 126 117 122 120 4.3 3.5% Sample 1 115 126 117 122 120 4.3 3.5% Sample 2 865 746 848 804 815 46.01 5.6% Sample 2 865 746 848 804 815 46.01 5.6% Sample 3 2242 2225 2256 2223 2236 13.46 0.6% Sample 3 2242 2225 2256 2223 2236 13.46 0.6%
- Synonyms
- Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD254; Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form; TNFSF11; OPGL, RANKL, TRANCE
- Gene Name
- TNFSF11
- Gene ID
- 8600
- UniProt
- O14788
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: G64-D245
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Atherosclerosis, Cardiovascular, Cytokines, Cytoskeleton/ECM, Extracellular Matrix, Immunology, Innate Immunity, Signal Transduction, Structures, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 78 pg/mL - 5,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 115 865 2242 114 797 2279 Standard deviation 5.52 53.63 105.37 4.72 58.97 116.22 CV (%) 4.8% 6.2% 4.7% 5.2% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 115 865 2242 114 797 2279 Standard deviation 5.52 53.63 105.37 4.72 58.97 116.22 CV (%) 4.8% 6.2% 4.7% 5.2% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 115 865 2242 114 797 2279 Mean (pg/ml) 115 865 2242 114 797 2279 Standard deviation 5.52 53.63 105.37 4.72 58.97 116.22 Standard deviation 5.52 53.63 105.37 4.72 58.97 116.22 CV (%) 4.8% 6.2% 4.7% 5.2% 7.4% 5.1% CV (%) 4.8% 6.2% 4.7% 5.2% 7.4% 5.1%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 35478 MW
- Specificity
- Natural and recombinant human TNFSF11
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor ligand superfamily member 11
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type II membrane protein.
- Protein Name
- Tumor necrosis factor ligand superfamily member 11
- Sample Data
- Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.043 0.106 0.145 0.279 0.483 0.851 1.501 2.046 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.043 0.106 0.145 0.279 0.483 0.851 1.501 2.046 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.043 0.106 0.145 0.279 0.483 0.851 1.501 2.046 O.D. 0.043 0.106 0.145 0.279 0.483 0.851 1.501 2.046
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
