- Catalog number
- EK0843
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 39 32 37 35 2.69 7.6% Sample 2 195 191 201 186 193 5.49 2.8% Sample 3 430 475 480 482 466 21.37 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 39 32 37 35 2.69 7.6% Sample 1 34 39 32 37 35 2.69 7.6% Sample 2 195 191 201 186 193 5.49 2.8% Sample 2 195 191 201 186 193 5.49 2.8% Sample 3 430 475 480 482 466 21.37 4.5% Sample 3 430 475 480 482 466 21.37 4.5%
- Synonyms
- Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD254; Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form; Tnfsf11; Opgl, Rankl, Trance
- Gene Name
- TNFSF11
- Gene ID
- 21943
- UniProt
- O35235
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: R72-D316
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Highly expressed in thymus and lymph nodes, but not in non-lymphoid tissues and is abundantly expressed in T- cells but not in B-cells. A high level expression is also seen in the trabecular bone and lung.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Atherosclerosis, Cardiovascular, Cytokines, Cytoskeleton/ECM, Extracellular Matrix, Immunology, Innate Immunity, Signal Transduction, Structures, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 132 430 32 199 419 Standard deviation 1.42 10.72 17.63 1.72 12.13 21.36 CV (%) 4.2% 5.5% 4.1% 5.4% 6.1% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 132 430 32 199 419 Standard deviation 1.42 10.72 17.63 1.72 12.13 21.36 CV (%) 4.2% 5.5% 4.1% 5.4% 6.1% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 132 430 32 199 419 Mean (pg/ml) 34 132 430 32 199 419 Standard deviation 1.42 10.72 17.63 1.72 12.13 21.36 Standard deviation 1.42 10.72 17.63 1.72 12.13 21.36 CV (%) 4.2% 5.5% 4.1% 5.4% 6.1% 5.1% CV (%) 4.2% 5.5% 4.1% 5.4% 6.1% 5.1%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 35003 MW
- Specificity
- Natural and recombinant mouse TNFSF11
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor ligand superfamily member 11
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type II membrane protein.
- Protein Name
- Tumor necrosis factor ligand superfamily member 11
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.094 0.162 0.314 0.561 0.973 1.544 2.063 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.094 0.162 0.314 0.561 0.973 1.544 2.063 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.094 0.162 0.314 0.561 0.973 1.544 2.063 O.D. 0.006 0.094 0.162 0.314 0.561 0.973 1.544 2.063
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
