- Catalog number
- EK0850
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 580 586 560 663 597 39.16 6.5% Sample 2 2272 2188 2044 2304 2202 100.57 4.5% Sample 3 9558 8956 8970 10074 9389 463.9 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 580 586 560 663 597 39.16 6.5% Sample 1 580 586 560 663 597 39.16 6.5% Sample 2 2272 2188 2044 2304 2202 100.57 4.5% Sample 2 2272 2188 2044 2304 2202 100.57 4.5% Sample 3 9558 8956 8970 10074 9389 463.9 4.9% Sample 3 9558 8956 8970 10074 9389 463.9 4.9%
- Synonyms
- Myeloperoxidase; MPO;1.11.2.2; Myeloperoxidase;89 kDa myeloperoxidase;84 kDa myeloperoxidase; Myeloperoxidase light chain; Myeloperoxidase heavy chain; MPO
- Gene Name
- MPO
- Gene ID
- 4353
- UniProt
- P05164
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A49-S745
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cancer Metabolism, Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Pathways and Processes, Redox Metabolism, Vasculature
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA), saliva and urine.
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 580 2272 9558 587 2421 9230 Standard deviation 42.92 154.49 401.43 48.13 191.25 507.65 CV (%) 7.4% 6.8% 4.2% 8.2% 7.9% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 580 2272 9558 587 2421 9230 Standard deviation 42.92 154.49 401.43 48.13 191.25 507.65 CV (%) 7.4% 6.8% 4.2% 8.2% 7.9% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 580 2272 9558 587 2421 9230 Mean (pg/ml) 580 2272 9558 587 2421 9230 Standard deviation 42.92 154.49 401.43 48.13 191.25 507.65 Standard deviation 42.92 154.49 401.43 48.13 191.25 507.65 CV (%) 7.4% 6.8% 4.2% 8.2% 7.9% 5.5% CV (%) 7.4% 6.8% 4.2% 8.2% 7.9% 5.5%
- Sensitivity
- <10 pg/mL
- Function
- Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 83869 MW
- Specificity
- Natural and recombinant human MPO
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Myeloperoxidase
- Subcellular Location
- Lysosome.
- Sequence Similarities
- Belongs to the peroxidase family. XPO subfamily.
- Protein Name
- Myeloperoxidase
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.015 0.117 0.2211 0.3838 0.6726 1.234 2.0 2.861 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.015 0.117 0.2211 0.3838 0.6726 1.234 2.0 2.861 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.015 0.117 0.2211 0.3838 0.6726 1.234 2.0 2.861 O.D. 0.015 0.117 0.2211 0.3838 0.6726 1.234 2.0 2.861
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
