- Catalog number
- EK0852
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), CD10, and common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 165 178 186 177 176 7.5 4.2% Sample 2 1021 907 995 1027 987 48 4.8% Sample 3 3228 3394 3345 3463 3357 85.71 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 165 178 186 177 176 7.5 4.2% Sample 1 165 178 186 177 176 7.5 4.2% Sample 2 1021 907 995 1027 987 48 4.8% Sample 2 1021 907 995 1027 987 48 4.8% Sample 3 3228 3394 3345 3463 3357 85.71 2.5% Sample 3 3228 3394 3345 3463 3357 85.71 2.5%
- Synonyms
- Neprilysin;3.4.24.11 ; Atriopeptidase; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD10; Mme
- Gene Name
- MME
- Gene ID
- 17380
- UniProt
- Q61391
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Y52-W750
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 93.7 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 165 1021 3228 175 951 3447 Standard deviation 7.26 41.86 216.27 4.7%2 57.4%5 255.07 CV (%) 4.4% 4.1% 6.7% 4.7% 5.8% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 165 1021 3228 175 951 3447 Standard deviation 7.26 41.86 216.27 4.7%2 57.4%5 255.07 CV (%) 4.4% 4.1% 6.7% 4.7% 5.8% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 165 1021 3228 175 951 3447 Mean (pg/ml) 165 1021 3228 175 951 3447 Standard deviation 7.26 41.86 216.27 4.7%2 57.4%5 255.07 Standard deviation 7.26 41.86 216.27 4.7%2 57.4%5 255.07 CV (%) 4.4% 4.1% 6.7% 4.7% 5.8% 7.4% CV (%) 4.4% 4.1% 6.7% 4.7% 5.8% 7.4%
- Sensitivity
- <10 pg/mL
- Function
- Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) (By similarity) . Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 85702 MW
- Specificity
- Natural and recombinant mouse CD10
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Neprilysin
- Subcellular Location
- Cell membrane; Single-pass type II membrane protein.
- Protein Name
- Neprilysin
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.065 0.142 0.211 0.33 0.561 1.013 1.748 2.237 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.065 0.142 0.211 0.33 0.561 1.013 1.748 2.237 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.065 0.142 0.211 0.33 0.561 1.013 1.748 2.237 O.D. 0.065 0.142 0.211 0.33 0.561 1.013 1.748 2.237
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
