- Catalog number
- EK0857
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 197 197 202 201 4.91 2.4% Sample 2 1225 1132 1200 1122 1169 43.79 3.7% Sample 3 4641 5209 4997 5343 5047 265.13 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 197 197 202 201 4.91 2.4% Sample 1 209 197 197 202 201 4.91 2.4% Sample 2 1225 1132 1200 1122 1169 43.79 3.7% Sample 2 1225 1132 1200 1122 1169 43.79 3.7% Sample 3 4641 5209 4997 5343 5047 265.13 5.2% Sample 3 4641 5209 4997 5343 5047 265.13 5.2%
- Synonyms
- Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; CD252; TNFSF4; TXGP1
- Gene Name
- TNFSF4
- Gene ID
- 7292
- UniProt
- P23510
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q51-L183
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Autoimmune, B Cells, Cell Type Markers, Cytokines, Immune System Diseases, Immunology, Innate Immunity, T Cells, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 1225 4641 228 1277 4885 Standard deviation 8.56 51.45 305.70 12.54 72.78 365.77 CV (%) 4.1% 4.2% 6.6% 5.5% 5.7% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 1225 4641 228 1277 4885 Standard deviation 8.56 51.45 305.70 12.54 72.78 365.77 CV (%) 4.1% 4.2% 6.6% 5.5% 5.7% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 209 1225 4641 228 1277 4885 Mean (pg/ml) 209 1225 4641 228 1277 4885 Standard deviation 8.56 51.45 305.70 12.54 72.78 365.77 Standard deviation 8.56 51.45 305.70 12.54 72.78 365.77 CV (%) 4.1% 4.2% 6.6% 5.5% 5.7% 7.5% CV (%) 4.1% 4.2% 6.6% 5.5% 5.7% 7.5%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 21050 MW
- Specificity
- Natural and recombinant human TNFSF4
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor ligand superfamily member 4
- Subcellular Location
- Membrane; Single-pass type II membrane protein.
- Sequence Similarities
- Belongs to the tumor necrosis factor family.
- Protein Name
- Tumor necrosis factor ligand superfamily member 4
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.101 0.19 0.316 0.557 1.018 1.751 2.543 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.101 0.19 0.316 0.557 1.018 1.751 2.543 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.101 0.19 0.316 0.557 1.018 1.751 2.543 O.D. 0.025 0.101 0.19 0.316 0.557 1.018 1.751 2.543
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
