- Catalog number
- EK0858
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, Baum et al. (1994) mapped the mouse Tnfsf4 gene to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene.OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 176 174 151 149 162 12.53 7.7% Sample 2 562 596 510 588 564 33.61 5.9% Sample 3 2082 1768 1972 2071 1973 126 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 176 174 151 149 162 12.53 7.7% Sample 1 176 174 151 149 162 12.53 7.7% Sample 2 562 596 510 588 564 33.61 5.9% Sample 2 562 596 510 588 564 33.61 5.9% Sample 3 2082 1768 1972 2071 1973 126 6.3% Sample 3 2082 1768 1972 2071 1973 126 6.3%
- Synonyms
- Tumor necrosis factor ligand superfamily member 4; OX40 ligand; OX40L; CD252; Tnfsf4; Ox40l, Txgp1l
- Gene Name
- TNFSF4
- Gene ID
- 22164
- UniProt
- P43488
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S51-L198
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Autoimmune, B Cells, Cell Type Markers, Cytokines, Immune System Diseases, Immunology, Innate Immunity, T Cells, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 176 562 2082 160 512 2260 Standard deviation 10.38 36.81 141.57 10.88 38.91 187.58 CV (%) 6.8% 6.8% 6.8% 6.8% 7.6% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 176 562 2082 160 512 2260 Standard deviation 10.38 36.81 141.57 10.88 38.91 187.58 CV (%) 6.8% 6.8% 6.8% 6.8% 7.6% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 176 562 2082 160 512 2260 Mean (pg/ml) 176 562 2082 160 512 2260 Standard deviation 10.38 36.81 141.57 10.88 38.91 187.58 Standard deviation 10.38 36.81 141.57 10.88 38.91 187.58 CV (%) 6.8% 6.8% 6.8% 6.8% 7.6% 8.3% CV (%) 6.8% 6.8% 6.8% 6.8% 7.6% 8.3%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 22255 MW
- Specificity
- Natural and recombinant mouse TNFSF4
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor ligand superfamily member 4
- Subcellular Location
- Membrane; Single-pass type II membrane protein.
- Protein Name
- Tumor necrosis factor ligand superfamily member 4
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.005 0.039 0.083 0.223 0.426 0.772 1.381 2.005 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.005 0.039 0.083 0.223 0.426 0.772 1.381 2.005 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.005 0.039 0.083 0.223 0.426 0.772 1.381 2.005 O.D. 0.005 0.039 0.083 0.223 0.426 0.772 1.381 2.005
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
