- Catalog number
- EK0859
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots) . It is a serine protease inhibitor (serpin) protein (SERPINE1) . PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome (7q21.3-q22) .1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency to hemorrhage) . PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 218 218 234 218 10.96 5% Sample 2 1208 1308 1324 1403 1310 69.37 5.2% Sample 3 5496 5184 6077 5696 5613 324 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 218 218 234 218 10.96 5% Sample 1 203 218 218 234 218 10.96 5% Sample 2 1208 1308 1324 1403 1310 69.37 5.2% Sample 2 1208 1308 1324 1403 1310 69.37 5.2% Sample 3 5496 5184 6077 5696 5613 324 5.7% Sample 3 5496 5184 6077 5696 5613 324 5.7%
- Synonyms
- Plasminogen activator inhibitor 1; PAI; PAI-1; Endothelial plasminogen activator inhibitor; Serpin E1; SERPINE1; PAI1, PLANH1
- Gene Name
- SERPINE1
- Gene ID
- 5054
- UniProt
- P05121
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S22-P402
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cardiovascular
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 1208 5496 198 1244 5980 Standard deviation 12.38 71.27 423.19 15.04 95.78 520.26 CV (%) 6.1% 7.7%% 7.7% 7.6% 7.7% 8.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 1208 5496 198 1244 5980 Standard deviation 12.38 71.27 423.19 15.04 95.78 520.26 CV (%) 6.1% 7.7%% 7.7% 7.6% 7.7% 8.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 203 1208 5496 198 1244 5980 Mean (pg/ml) 203 1208 5496 198 1244 5980 Standard deviation 12.38 71.27 423.19 15.04 95.78 520.26 Standard deviation 12.38 71.27 423.19 15.04 95.78 520.26 CV (%) 6.1% 7.7%% 7.7% 7.6% 7.7% 8.7% CV (%) 6.1% 7.7%% 7.7% 7.6% 7.7% 8.7%
- Sensitivity
- <10 pg/mL
- Function
- Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 45060 MW
- Specificity
- Natural and recombinant human PAI-1
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Plasminogen activator inhibitor 1
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the serpin family.
- Protein Name
- Plasminogen activator inhibitor 1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.138 0.221 0.406 0.712 1.466 2.268 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.138 0.221 0.406 0.712 1.466 2.268 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.138 0.221 0.406 0.712 1.466 2.268 O.D. 0.055 0.101 0.138 0.221 0.406 0.712 1.466 2.268
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
