Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®

About this product

Human IL-18 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0864

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin (IL) -18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 32 33 31 32 0.82 2.5% Sample 2 247 212 221 232 228 13.05 5.7% Sample 3 520 533 562 546 478 31.15 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 32 33 31 32 0.82 2.5% Sample 1 33 32 33 31 32 0.82 2.5% Sample 2 247 212 221 232 228 13.05 5.7% Sample 2 247 212 221 232 228 13.05 5.7% Sample 3 520 533 562 546 478 31.15 5.4% Sample 3 520 533 562 546 478 31.15 5.4%

Synonyms

Interleukin-18; IL-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; IL18; IGIF, IL1F4

Gene Name

IL18

Gene ID

3606

UniProt

Q14116

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Y33-D197

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Monoclonal antibody from mouse

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cancer Metabolism, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 247 520 36 254 548 Standard deviation 2.4 13.83 21.84 2.66 16.25 31.78 CV (%) 7.3% 5.6% 4.2% 7.4% 6.4% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 247 520 36 254 548 Standard deviation 2.4 13.83 21.84 2.66 16.25 31.78 CV (%) 7.3% 5.6% 4.2% 7.4% 6.4% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 247 520 36 254 548 Mean (pg/ml) 33 247 520 36 254 548 Standard deviation 2.4 13.83 21.84 2.66 16.25 31.78 Standard deviation 2.4 13.83 21.84 2.66 16.25 31.78 CV (%) 7.3% 5.6% 4.2% 7.4% 6.4% 5.8% CV (%) 7.3% 5.6% 4.2% 7.4% 6.4% 5.8%

Sensitivity

<1 pg/mL

Function

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22326 MW

Specificity

Natural and recombinant human IL-18

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-18

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-1 family.

Protein Name

Interleukin-18

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.06 0.104 0.183 0.312 0.594 1.111 1.932 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.06 0.104 0.183 0.312 0.594 1.111 1.932 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.06 0.104 0.183 0.312 0.594 1.111 1.932 O.D. 0.017 0.06 0.104 0.183 0.312 0.594 1.111 1.932

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.