- Catalog number
- EK0866
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-23 subunit alpha (IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit) . IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812) .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 84 99 94 92 5.4 5.8% Sample 2 278 287 274 301 285 10.36 3.6% Sample 3 780 941 844 875 860 57.96 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 84 99 94 92 5.4 5.8% Sample 1 93 84 99 94 92 5.4 5.8% Sample 2 278 287 274 301 285 10.36 3.6% Sample 2 278 287 274 301 285 10.36 3.6% Sample 3 780 941 844 875 860 57.96 6.7% Sample 3 780 941 844 875 860 57.96 6.7%
- Synonyms
- Interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; Interleukin-23 subunit p19; IL-23p19; Il23a
- Gene Name
- IL23A
- Gene ID
- 83430
- UniProt
- Q9EQ14
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: (M23-S335) + (L20-A196)
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Secreted by activated dendritic cells (at protein level) . Detected in various tissues with higher expression in polarized Th1 cells and activated macrophages. .
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 278 780 85 279 762 Standard deviation 4.09 13.9 34.32 4.4%7 19.25 38.86 CV (%) 4.4% 5% 4.4% 6.2% 6.9% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 278 780 85 279 762 Standard deviation 4.09 13.9 34.32 4.4%7 19.25 38.86 CV (%) 4.4% 5% 4.4% 6.2% 6.9% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 93 278 780 85 279 762 Mean (pg/ml) 93 278 780 85 279 762 Standard deviation 4.09 13.9 34.32 4.4%7 19.25 38.86 Standard deviation 4.09 13.9 34.32 4.4%7 19.25 38.86 CV (%) 4.4% 5% 4.4% 6.2% 6.9% 5.1% CV (%) 4.4% 5% 4.4% 6.2% 6.9% 5.1%
- Sensitivity
- <2 pg/mL
- Function
- Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 22071 MW
- Specificity
- Natural and recombinant mouse IL-23
- Anticoagulant
- Heparin
- Gene Name Synonym
- Interleukin-23 subunit alpha
- Subcellular Location
- Secreted . Secreted upon association with IL12B.
- Protein Name
- Interleukin-23 subunit alpha
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.043 0.115 0.175 0.268 0.446 0.823 1.418 2.273 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.043 0.115 0.175 0.268 0.446 0.823 1.418 2.273 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.043 0.115 0.175 0.268 0.446 0.823 1.418 2.273 O.D. 0.043 0.115 0.175 0.268 0.446 0.823 1.418 2.273
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
