- Catalog number
- EK0867
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 125 119 115 116 6.17 5.3% Sample 2 660 564 616 629 617 34.65 5.6% Sample 3 1140 1087 1155 1110 1123 26.35 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 125 119 115 116 6.17 5.3% Sample 1 108 125 119 115 116 6.17 5.3% Sample 2 660 564 616 629 617 34.65 5.6% Sample 2 660 564 616 629 617 34.65 5.6% Sample 3 1140 1087 1155 1110 1123 26.35 2.3% Sample 3 1140 1087 1155 1110 1123 26.35 2.3%
- Synonyms
- Dickkopf-like protein 1 ; DKK1
- Gene Name
- DKK1
- Gene ID
- 22943
- UniProt
- I1W660
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: T32-H266
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cardiovascular, Developmental Biology, Embryogenesis, Immunology, Secreted, Signaling Pathways, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 660 1140 103 594 1134 Standard deviation 5.72 47.52 66.12 6.59 43.36 82.78 CV (%) 5.3% 7.2% 5.8% 6.4% 7.3% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 660 1140 103 594 1134 Standard deviation 5.72 47.52 66.12 6.59 43.36 82.78 CV (%) 5.3% 7.2% 5.8% 6.4% 7.3% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 660 1140 103 594 1134 Mean (pg/ml) 108 660 1140 103 594 1134 Standard deviation 5.72 47.52 66.12 6.59 43.36 82.78 Standard deviation 5.72 47.52 66.12 6.59 43.36 82.78 CV (%) 5.3% 7.2% 5.8% 6.4% 7.3% 7.3% CV (%) 5.3% 7.2% 5.8% 6.4% 7.3% 7.3%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 28672 MW
- Specificity
- Oligomeric forms of natural and recombinant human DKK1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Dickkopf WNT signaling pathway inhibitor 1
- Protein Name
- Dickkopf-related protein 1
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.07 0.113 0.218 0.358 0.628 1.048 1.96 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.07 0.113 0.218 0.358 0.628 1.048 1.96 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.07 0.113 0.218 0.358 0.628 1.048 1.96 O.D. 0.014 0.07 0.113 0.218 0.358 0.628 1.048 1.96
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
