Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine®

About this product

Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Pecam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0874

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a member of the immunoglobulin (Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs (ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor (TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2 (SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 563 amino acids (E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 217 221 199 216 10.7 4.9% Sample 2 1326 1359 1160 1320 1291 77.21 5.9% Sample 3 5663 6219 5814 5340 5759 315.99 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 217 221 199 216 10.7 4.9% Sample 1 228 217 221 199 216 10.7 4.9% Sample 2 1326 1359 1160 1320 1291 77.21 5.9% Sample 2 1326 1359 1160 1320 1291 77.21 5.9% Sample 3 5663 6219 5814 5340 5759 315.99 5.4% Sample 3 5663 6219 5814 5340 5759 315.99 5.4%

Synonyms

Platelet endothelial cell adhesion molecule; PECAM-1; CD31; Pecam1; Pecam, Pecam-1

Gene Name

PECAM1

Gene ID

18613

UniProt

Q08481

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-K590

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Isoform 1 and isoform 3 are expressed in lung and platelets. .

Applications

ELISA

Field of Research

Angiogenesis, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Immunology, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1326 5663 230 1398 5316 Standard deviation 17.32 74.25 407.73 18.17 99.25 409.33 CV (%) 7.6% 5.6% 7.2% 7.9% 7.1% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1326 5663 230 1398 5316 Standard deviation 17.32 74.25 407.73 18.17 99.25 409.33 CV (%) 7.6% 5.6% 7.2% 7.9% 7.1% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 228 1326 5663 230 1398 5316 Mean (pg/ml) 228 1326 5663 230 1398 5316 Standard deviation 17.32 74.25 407.73 18.17 99.25 409.33 Standard deviation 17.32 74.25 407.73 18.17 99.25 409.33 CV (%) 7.6% 5.6% 7.2% 7.9% 7.1% 7.7% CV (%) 7.6% 5.6% 7.2% 7.9% 7.1% 7.7%

Sensitivity

<10 pg/mL

Function

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment) . Modulates BDKRB2 activation (By similarity) . Induces susceptibility to atherosclerosis. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

81263 MW

Specificity

Natural and recombinant mouse PECAM-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Platelet endothelial cell adhesion molecule

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Cell membrane ; Lipid-anchor . Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. .

Sequence Similarities

Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Protein Name

Platelet endothelial cell adhesion molecule

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.018 0.036 0.056 0.089 0.171 0.339 0.755 1.719 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.018 0.036 0.056 0.089 0.171 0.339 0.755 1.719 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.018 0.036 0.056 0.089 0.171 0.339 0.755 1.719 O.D. 0.018 0.036 0.056 0.089 0.171 0.339 0.755 1.719

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.