- Catalog number
- EK0880
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 87 90 90 96 90 3.26 3.6% Sample 2 279 266 249 284 269 13.53 5% Sample 3 842 734 811 767 788 41.23 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 87 90 90 96 90 3.26 3.6% Sample 1 87 90 90 96 90 3.26 3.6% Sample 2 279 266 249 284 269 13.53 5% Sample 2 279 266 249 284 269 13.53 5% Sample 3 842 734 811 767 788 41.23 5.2% Sample 3 842 734 811 767 788 41.23 5.2%
- Synonyms
- Hepatitis A virus cellular receptor 1 homolog ; Hepatitis A virus cellular receptor 1, isoform CRA_b ; Havcr1 ; mCG_117142
- Gene Name
- HAVCR1
- Gene ID
- 171283
- UniProt
- Q3V033
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Y22-T212
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Host-Virus Interaction, Immune System Diseases, Immunology, Interspecies Interaction, Microbiology, Non-CD, T Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 87 279 842 85 261 792 Standard deviation 6.87 14.78 36.2 7.31 14.87 38.8 CV (%) 7.9% 5.3% 4.3% 8.6% 5.7% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 87 279 842 85 261 792 Standard deviation 6.87 14.78 36.2 7.31 14.87 38.8 CV (%) 7.9% 5.3% 4.3% 8.6% 5.7% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 87 279 842 85 261 792 Mean (pg/ml) 87 279 842 85 261 792 Standard deviation 6.87 14.78 36.2 7.31 14.87 38.8 Standard deviation 6.87 14.78 36.2 7.31 14.87 38.8 CV (%) 7.9% 5.3% 4.3% 8.6% 5.7% 4.9% CV (%) 7.9% 5.3% 4.3% 8.6% 5.7% 4.9%
- Sensitivity
- <2 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 30968 MW
- Specificity
- Natural and recombinant mouse KIM1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Hepatitis A virus cellular receptor 1
- Protein Name
- Hepatitis A virus cellular receptor 1 homolog
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 2.158 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 2.158 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 2.158 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 2.158
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
