- Catalog number
- EK0883
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rats is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to Rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the Rat Tim1 gene and database analysis, the HAVCR1 gene was mapped to 5q33.2.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 93 93 91 92 0.82 0.8% Sample 2 215 235 212 211 218 9.78 4.4% Sample 3 880 798 777 920 843 58.47 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 93 93 91 92 0.82 0.8% Sample 1 92 93 93 91 92 0.82 0.8% Sample 2 215 235 212 211 218 9.78 4.4% Sample 2 215 235 212 211 218 9.78 4.4% Sample 3 880 798 777 920 843 58.47 6.9% Sample 3 880 798 777 920 843 58.47 6.9%
- Synonyms
- Hepatitis A virus cellular receptor 1; HAVcr-1; Kidney injury molecule 1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; T-cell immunoglobulin mucin receptor 1; TIM; TIM-1; T-cell membrane protein 1; HAVCR1; KIM1, TIM1, TIMD1
- Gene Name
- HAVCR1
- Gene ID
- 26762
- UniProt
- Q96D42
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S21-T288
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. .
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Host-Virus Interaction, Immune System Diseases, Immunology, Interspecies Interaction, Microbiology, Non-CD, T Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 215 880 93 208 802 Standard deviation 5.42 9.24 39.6 5.48 11.02 40.1 CV (%) 4.5% 4.3% 4.5% 5.9% 5.3% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 215 880 93 208 802 Standard deviation 5.42 9.24 39.6 5.48 11.02 40.1 CV (%) 4.5% 4.3% 4.5% 5.9% 5.3% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 92 215 880 93 208 802 Mean (pg/ml) 92 215 880 93 208 802 Standard deviation 5.42 9.24 39.6 5.48 11.02 40.1 Standard deviation 5.42 9.24 39.6 5.48 11.02 40.1 CV (%) 4.5% 4.3% 4.5% 5.9% 5.3% 5% CV (%) 4.5% 4.3% 4.5% 5.9% 5.3% 5%
- Sensitivity
- <2 pg/mL
- Function
- May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity) . In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 38720 MW
- Specificity
- Natural and recombinant human KIM1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Hepatitis A virus cellular receptor 1
- Subcellular Location
- Membrane ; Single-pass type I membrane protein .
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. TIM family.
- Protein Name
- Hepatitis A virus cellular receptor 1
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.127 0.194 0.301 0.506 0.935 1.57 2.607 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.127 0.194 0.301 0.506 0.935 1.57 2.607 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.127 0.194 0.301 0.506 0.935 1.57 2.607 O.D. 0.045 0.127 0.194 0.301 0.506 0.935 1.57 2.607
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
