- Catalog number
- EK0884
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 49 57 56 54 3.2 5.9% Sample 2 263 286 255 285 272 13.55 4.9% Sample 3 982 921 916 948 941 26.23 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 49 57 56 54 3.2 5.9% Sample 1 56 49 57 56 54 3.2 5.9% Sample 2 263 286 255 285 272 13.55 4.9% Sample 2 263 286 255 285 272 13.55 4.9% Sample 3 982 921 916 948 941 26.23 2.7% Sample 3 982 921 916 948 941 26.23 2.7%
- Synonyms
- Bone morphogenetic protein 7 (Osteogenic protein 1), isoform CRA_b ; cDNA FLJ78019, highly similar to Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1), mRNA ; cDNA, FLJ92758, Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1) (BMP7), mRNA ; BMP7 ; hCG_40100
- Gene Name
- BMP7
- Gene ID
- 655
- UniProt
- A8K571
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S293-H431
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Growth Factors/Hormones, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathways, Metabolism, Nervous System Development, Organogenesis, Pathways and Processes, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, TGF Beta
- Standard
- 10 ng/Tube
- Sample Type
- Bone tissue, cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 263 982 53 244 937 Standard deviation 4.08 12.36 54.01 4.66 15.86 57.15 CV (%) 7.3% 4.7% 5.5% 8.8% 6.5% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 263 982 53 244 937 Standard deviation 4.08 12.36 54.01 4.66 15.86 57.15 CV (%) 7.3% 4.7% 5.5% 8.8% 6.5% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 56 263 982 53 244 937 Mean (pg/ml) 56 263 982 53 244 937 Standard deviation 4.08 12.36 54.01 4.66 15.86 57.15 Standard deviation 4.08 12.36 54.01 4.66 15.86 57.15 CV (%) 7.3% 4.7% 5.5% 8.8% 6.5% 6.1% CV (%) 7.3% 4.7% 5.5% 8.8% 6.5% 6.1%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 49313 MW
- Specificity
- Natural and recombinant human BMP-7
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Bone morphogenetic protein 7
- Protein Name
- Bone morphogenetic protein 7
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.103 0.182 0.328 0.581 1.016 1.822 2.935 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.103 0.182 0.328 0.581 1.016 1.822 2.935 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.103 0.182 0.328 0.581 1.016 1.822 2.935 O.D. 0.009 0.103 0.182 0.328 0.581 1.016 1.822 2.935
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
