- Catalog number
- EK0899
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2 (region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2414 2383 2098 2136 2257 141.81 6.2% Sample 2 13444 12251 11532 12803 12507 703.87 5.6% Sample 3 38908 44108 38626 45595 41809 3088.95 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2414 2383 2098 2136 2257 141.81 6.2% Sample 1 2414 2383 2098 2136 2257 141.81 6.2% Sample 2 13444 12251 11532 12803 12507 703.87 5.6% Sample 2 13444 12251 11532 12803 12507 703.87 5.6% Sample 3 38908 44108 38626 45595 41809 3088.95 7.3% Sample 3 38908 44108 38626 45595 41809 3088.95 7.3%
- Synonyms
- Thrombospondin-1; THBS1; TSP, TSP1
- Gene Name
- THBS1
- Gene ID
- 7057
- UniProt
- P07996
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: N19-P1170
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cell Adhesion Proteins, Cell Type Marker, Invasion/Microenvironment, Metabolism, Metabolism Processes, Neuroscience, Pathways and Processes, Platelets, Response To Hypoxia, Secreted, Signaling Pathways, Stem Cells, TGF Beta
- Standard
- 100 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 1.56 ng/mL - 100 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2414 13444 38908 2523 13902 38494 Standard deviation 152.08 994.85 2451.2 176.61 1278.98 2771.56 CV (%) 9.2%% 7.4% 9.2%% 7% 9.2% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2414 13444 38908 2523 13902 38494 Standard deviation 152.08 994.85 2451.2 176.61 1278.98 2771.56 CV (%) 9.2%% 7.4% 9.2%% 7% 9.2% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2414 13444 38908 2523 13902 38494 Mean (pg/ml) 2414 13444 38908 2523 13902 38494 Standard deviation 152.08 994.85 2451.2 176.61 1278.98 2771.56 Standard deviation 152.08 994.85 2451.2 176.61 1278.98 2771.56 CV (%) 9.2%% 7.4% 9.2%% 7% 9.2% 7.2% CV (%) 9.2%% 7.4% 9.2%% 7% 9.2% 7.2%
- Sensitivity
- <50 pg/mL
- Function
- Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity) . Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 129383 MW
- Specificity
- Natural and recombinant human THBS1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Thrombospondin-1
- Subcellular Location
- Endoplasmic reticulum . Sarcoplasmic reticulum .
- Sequence Similarities
- Belongs to the thrombospondin family.
- Protein Name
- Thrombospondin-1
- Sample Data
- Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.029 0.098 0.157 0.298 0.574 0.994 1.689 2.367 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.029 0.098 0.157 0.298 0.574 0.994 1.689 2.367 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.029 0.098 0.157 0.298 0.574 0.994 1.689 2.367 O.D. 0.029 0.098 0.157 0.298 0.574 0.994 1.689 2.367
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
