Rat IL-13/Interleukin-13 ELISA Kit PicoKine®

About this product

Rat IL-13 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Il13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0900

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ralpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6) . The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. Physical maps of the 5q23-q31 region show the following order: cen--IL3--CSF2--IL13--IL4--IL5--tel, with IL13 particularly close to IL4.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 37 37 37 37 0 0% Sample 2 170 186 180 173 177 6.21 3.5% Sample 3 412 389 434 402 409 16.45 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 37 37 37 37 0 0% Sample 1 37 37 37 37 37 0 0% Sample 2 170 186 180 173 177 6.21 3.5% Sample 2 170 186 180 173 177 6.21 3.5% Sample 3 412 389 434 402 409 16.45 4% Sample 3 412 389 434 402 409 16.45 4%

Synonyms

Interleukin-13; IL-13; T-cell activation protein P600; Il13; Il-13

Gene Name

IL13

Gene ID

116553

UniProt

P42203

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T19-H131

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 170 412 36 164 394 Standard deviation 2.73 10.37 22.66 3.09 12.95 27.58 CV (%) 7.4% 6.1% 5.5% 8.6% 7.9% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 170 412 36 164 394 Standard deviation 2.73 10.37 22.66 3.09 12.95 27.58 CV (%) 7.4% 6.1% 5.5% 8.6% 7.9% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 170 412 36 164 394 Mean (pg/ml) 37 170 412 36 164 394 Standard deviation 2.73 10.37 22.66 3.09 12.95 27.58 Standard deviation 2.73 10.37 22.66 3.09 12.95 27.58 CV (%) 7.4% 6.1% 5.5% 8.6% 7.9% 7% CV (%) 7.4% 6.1% 5.5% 8.6% 7.9% 7%

Sensitivity

<1 pg/mL

Function

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses (By similarity) . .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

14093 MW

Specificity

Natural and recombinant rat IL-13

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-13

Subcellular Location

Secreted.

Protein Name

Interleukin-13

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.002 0.062 0.127 0.246 0.436 0.787 1.246 1.831 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.002 0.062 0.127 0.246 0.436 0.787 1.246 1.831 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.002 0.062 0.127 0.246 0.436 0.787 1.246 1.831 O.D. 0.002 0.062 0.127 0.246 0.436 0.787 1.246 1.831

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.