- Catalog number
- EK0903
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 305 265 265 284 19.75 6.9% Sample 2 1654 1642 1403 1384 1520 127.49 8.3% Sample 3 3697 4059 4071 4283 4027 210.59 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 305 265 265 284 19.75 6.9% Sample 1 304 305 265 265 284 19.75 6.9% Sample 2 1654 1642 1403 1384 1520 127.49 8.3% Sample 2 1654 1642 1403 1384 1520 127.49 8.3% Sample 3 3697 4059 4071 4283 4027 210.59 5.2% Sample 3 3697 4059 4071 4283 4027 210.59 5.2%
- Synonyms
- Kallikrein-1;3.4.21.35; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein; KLK1
- Gene Name
- KLK1
- Gene ID
- 3816
- UniProt
- P06870
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: I25-S262
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. .
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Serum Proteins, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1654 3697 280 1605 3442 Standard deviation 12.46 71.12 284.66 16.24 93.09 271.91 CV (%) 4.1% 4.3% 7.7% 5.8% 5.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1654 3697 280 1605 3442 Standard deviation 12.46 71.12 284.66 16.24 93.09 271.91 CV (%) 4.1% 4.3% 7.7% 5.8% 5.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 304 1654 3697 280 1605 3442 Mean (pg/ml) 304 1654 3697 280 1605 3442 Standard deviation 12.46 71.12 284.66 16.24 93.09 271.91 Standard deviation 12.46 71.12 284.66 16.24 93.09 271.91 CV (%) 4.1% 4.3% 7.7% 5.8% 5.8% 7.9% CV (%) 4.1% 4.3% 7.7% 5.8% 5.8% 7.9%
- Sensitivity
- <10 pg/mL
- Function
- Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 28890 MW
- Specificity
- Natural and recombinant human KLK1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Kallikrein-1
- Sequence Similarities
- Belongs to the peptidase S1 family. Kallikrein subfamily.
- Protein Name
- Kallikrein-1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.087 0.169 0.306 0.5438 0.938 1.364 1.849 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.087 0.169 0.306 0.5438 0.938 1.364 1.849 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.087 0.169 0.306 0.5438 0.938 1.364 1.849 O.D. 0.017 0.087 0.169 0.306 0.5438 0.938 1.364 1.849
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
