Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine®

About this product

Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine® (96 Tests) . Quantitate Human CEACAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0904

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 776 816 775 762 782 20.25 2.5% Sample 2 1668 1752 1723 1646 1697 42.25 2.4% Sample 3 10176 9323 9418 9597 9628 331.05 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 776 816 775 762 782 20.25 2.5% Sample 1 776 816 775 762 782 20.25 2.5% Sample 2 1668 1752 1723 1646 1697 42.25 2.4% Sample 2 1668 1752 1723 1646 1697 42.25 2.4% Sample 3 10176 9323 9418 9597 9628 331.05 3.4% Sample 3 10176 9323 9418 9597 9628 331.05 3.4%

Synonyms

Carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen; CEA; Meconium antigen 100; CD66e; CEACAM5; CEA

Gene Name

CEACAM5

Gene ID

111518

UniProt

P06731

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K35-A685

Capture Antibody

Polyclonal antibody from rabbit

Detection Antibody

Polyclonal antibody from rabbit

Tissue Specificity

Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

Applications

ELISA

Field of Research

Tumor Suppressors, Tumor-Associated Antigens

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 776 1668 10176 724 1662 10501 Standard deviation 39.57 124 407.04 39.09 137.94 420.04 CV (%) 4% 7.5% 4% 5.4% 8.3% 4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 776 1668 10176 724 1662 10501 Standard deviation 39.57 124 407.04 39.09 137.94 420.04 CV (%) 4% 7.5% 4% 5.4% 8.3% 4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 776 1668 10176 724 1662 10501 Mean (pg/ml) 776 1668 10176 724 1662 10501 Standard deviation 39.57 124 407.04 39.09 137.94 420.04 Standard deviation 39.57 124 407.04 39.09 137.94 420.04 CV (%) 4% 7.5% 4% 5.4% 8.3% 4% CV (%) 4% 7.5% 4% 5.4% 8.3% 4%

Sensitivity

<10 pg/mL

Function

Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

76795 MW

Specificity

Natural and recombinant human CEA

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Carcinoembryonic antigen-related cell adhesion molecule 5

Subcellular Location

Cell membrane ; Lipid-anchor, GPI-anchor .

Sequence Similarities

Belongs to the immunoglobulin superfamily. CEA family.

Protein Name

Carcinoembryonic antigen-related cell adhesion molecule 5

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.041 0.089 0.132 0.281 0.432 0.87 1.334 2.05 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.041 0.089 0.132 0.281 0.432 0.87 1.334 2.05 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.041 0.089 0.132 0.281 0.432 0.87 1.334 2.05 O.D. 0.041 0.089 0.132 0.281 0.432 0.87 1.334 2.05

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.