Human TLR2/Toll-like receptor 2 ELISA Kit PicoKine®

About this product

Human TLR2/Toll-like receptor 2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human TLR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0906

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Toll-like receptor 2 also known as TLR-2 is a protein that in humans is encoded by the TLR2 gene. TLR2 has also been designated as CD282 (cluster of differentiation 282) . TLR-2 plays a role in the immune system. TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle..

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 369 373 338 372 363 14.5 3.9% Sample 2 2018 1834 1789 2014 1913 103.49 5.4% Sample 3 5303 5820 5768 5756 5661 208.51 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 369 373 338 372 363 14.5 3.9% Sample 1 369 373 338 372 363 14.5 3.9% Sample 2 2018 1834 1789 2014 1913 103.49 5.4% Sample 2 2018 1834 1789 2014 1913 103.49 5.4% Sample 3 5303 5820 5768 5756 5661 208.51 3.6% Sample 3 5303 5820 5768 5756 5661 208.51 3.6%

Synonyms

Toll-like receptor

Gene Name

TLR2

Gene ID

7097

UniProt

O60603

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-L590

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adapters, Atherosclerosis, Cardiovascular, Defensin, Human Protein, Immunoglobulins, Immunology, Innate Immunity, Macrophage/Inflammation, Microbiology, Protein, Receptors, Signal Transduction, TLR Signaling, Transmembrane, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 369 2018 5303 392 1927 5643 Standard deviation 18.81 133.18 212.12 22.34 150.3 242.64 CV (%) 4.3% 6.6% 4% 5.7% 7.8% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 369 2018 5303 392 1927 5643 Standard deviation 18.81 133.18 212.12 22.34 150.3 242.64 CV (%) 4.3% 6.6% 4% 5.7% 7.8% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 369 2018 5303 392 1927 5643 Mean (pg/ml) 369 2018 5303 392 1927 5643 Standard deviation 18.81 133.18 212.12 22.34 150.3 242.64 Standard deviation 18.81 133.18 212.12 22.34 150.3 242.64 CV (%) 4.3% 6.6% 4% 5.7% 7.8% 4.3% CV (%) 4.3% 6.6% 4% 5.7% 7.8% 4.3%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

89826 MW

Specificity

Natural and recombinant human TLR2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Toll-like receptor

Sequence Similarities

Belongs to the Toll-like receptor family.

Protein Name

Toll-like receptor 2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.059 0.11 0.232 0.404 0.831 1.479 2.061 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.059 0.11 0.232 0.404 0.831 1.479 2.061 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.059 0.11 0.232 0.404 0.831 1.479 2.061 O.D. 0.004 0.059 0.11 0.232 0.404 0.831 1.479 2.061

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.