- Catalog number
- EK0912
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD23, also known as Fc epsilon RII, or FcεRII, is the low-affinity receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 71 62 62 64 4.06 6.3% Sample 2 194 218 229 235 219 15.66 7.1% Sample 3 981 870 950 851 913 54.05 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 71 62 62 64 4.06 6.3% Sample 1 61 71 62 62 64 4.06 6.3% Sample 2 194 218 229 235 219 15.66 7.1% Sample 2 194 218 229 235 219 15.66 7.1% Sample 3 981 870 950 851 913 54.05 5.9% Sample 3 981 870 950 851 913 54.05 5.9%
- Synonyms
- Low affinity immunoglobulin epsilon Fc receptor ; FCER2
- Gene Name
- FCER2
- Gene ID
- 2208
- UniProt
- K3W4U1
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D48-S321
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Receptors, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 194 981 55 207 899 Standard deviation 2.8 7.95 64.74 3.41 9.93 71.02 CV (%) 4.6% 4.1% 6.6% 6.2% 4.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 194 981 55 207 899 Standard deviation 2.8 7.95 64.74 3.41 9.93 71.02 CV (%) 4.6% 4.1% 6.6% 6.2% 4.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 61 194 981 55 207 899 Mean (pg/ml) 61 194 981 55 207 899 Standard deviation 2.8 7.95 64.74 3.41 9.93 71.02 Standard deviation 2.8 7.95 64.74 3.41 9.93 71.02 CV (%) 4.6% 4.1% 6.6% 6.2% 4.8% 7.9% CV (%) 4.6% 4.1% 6.6% 6.2% 4.8% 7.9%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 36299 MW
- Specificity
- Natural and recombinant human CD23
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Fc fragment of IgE, low affinity II, receptor for (CD23)
- Sequence Similarities
- Contains C-type lectin domain.
- Protein Name
- Low affinity immunoglobulin epsilon Fc receptor
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.056 0.108 0.199 0.356 0.608 1.155 2.001 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.056 0.108 0.199 0.356 0.608 1.155 2.001 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.056 0.108 0.199 0.356 0.608 1.155 2.001 O.D. 0.002 0.056 0.108 0.199 0.356 0.608 1.155 2.001
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
