- Catalog number
- EK0917
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor (TAFI, aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene (THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome (aHUS) .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 164 165 162 171 165 3.35 2% Sample 2 418 416 394 403 407 9.8 2.4% Sample 3 1319 1304 1439 1459 1380 69.31 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 164 165 162 171 165 3.35 2% Sample 1 164 165 162 171 165 3.35 2% Sample 2 418 416 394 403 407 9.8 2.4% Sample 2 418 416 394 403 407 9.8 2.4% Sample 3 1319 1304 1439 1459 1380 69.31 5% Sample 3 1319 1304 1439 1459 1380 69.31 5%
- Synonyms
- Thrombomodulin; TM; Fetomodulin; CD141; THBD; THRM
- Gene Name
- THBD
- Gene ID
- 7056
- UniProt
- P07204
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A19-S515
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Endothelial cells are unique in synthesizing thrombomodulin.
- Applications
- ELISA
- Field of Research
- Cardiovascular, Stem Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 164 418 1319 176 453 1243 Standard deviation 7.38 26.33 79.14 9.68 33.52 91.98 CV (%) 4.5% 6.3% 6% 5.5% 7.4% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 164 418 1319 176 453 1243 Standard deviation 7.38 26.33 79.14 9.68 33.52 91.98 CV (%) 4.5% 6.3% 6% 5.5% 7.4% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 164 418 1319 176 453 1243 Mean (pg/ml) 164 418 1319 176 453 1243 Standard deviation 7.38 26.33 79.14 9.68 33.52 91.98 Standard deviation 7.38 26.33 79.14 9.68 33.52 91.98 CV (%) 4.5% 6.3% 6% 5.5% 7.4% 7.4% CV (%) 4.5% 6.3% 6% 5.5% 7.4% 7.4%
- Sensitivity
- <10 pg/mL
- Function
- Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca) . Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 60329 MW
- Specificity
- Natural and recombinant human Thrombomodulin
- Anticoagulant
- Heparin, EDTA or Citrate
- Gene Name Synonym
- Thrombomodulin
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 1 C-type lectin domain.
- Protein Name
- Thrombomodulin
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.029 0.092 0.148 0.267 0.477 0.91 1.518 2.094 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.029 0.092 0.148 0.267 0.477 0.91 1.518 2.094 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.029 0.092 0.148 0.267 0.477 0.91 1.518 2.094 O.D. 0.029 0.092 0.148 0.267 0.477 0.91 1.518 2.094
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
