- Catalog number
- EK0921
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tumor necrosis factor ligand superfamily member 13 (TNFSF13) also known as a proliferation-inducing ligand (APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256 (cluster of differentiation 256) . The protein encoded by this gene is a member of the tumor necrosis factor ligand (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 265 272 285 271 8.38 3% Sample 2 1700 1548 1563 1481 1573 79.55 5% Sample 3 4235 4377 4466 4871 4487 236.37 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 265 272 285 271 8.38 3% Sample 1 264 265 272 285 271 8.38 3% Sample 2 1700 1548 1563 1481 1573 79.55 5% Sample 2 1700 1548 1563 1481 1573 79.55 5% Sample 3 4235 4377 4466 4871 4487 236.37 5.2% Sample 3 4235 4377 4466 4871 4487 236.37 5.2%
- Synonyms
- Tumor necrosis factor (Ligand) superfamily member 13 transcript variant delta ; Tumor necrosis factor ligand superfamily member 13 ; TNFSF13
- Gene Name
- TNFSF13
- Gene ID
- 8741
- UniProt
- Q2QBA2
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A105-L250
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Monoclonal antibody from mouse
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, B Cells, Cancer, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Non-CD, Signal Transduction, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 1700 4235 241 1784 4408 Standard deviation 14.25 119 237.16 15.42 144.5 317.37 CV (%) 5.4% 7% 5.6% 6.4% 8.1% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 1700 4235 241 1784 4408 Standard deviation 14.25 119 237.16 15.42 144.5 317.37 CV (%) 5.4% 7% 5.6% 6.4% 8.1% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 264 1700 4235 241 1784 4408 Mean (pg/ml) 264 1700 4235 241 1784 4408 Standard deviation 14.25 119 237.16 15.42 144.5 317.37 Standard deviation 14.25 119 237.16 15.42 144.5 317.37 CV (%) 5.4% 7% 5.6% 6.4% 8.1% 7.2% CV (%) 5.4% 7% 5.6% 6.4% 8.1% 7.2%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 24279 MW
- Specificity
- Natural and recombinant human TNFSF13
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor (ligand) superfamily, member 13
- Protein Name
- Tumor necrosis factor ligand superfamily member 13
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.14 0.21 0.311 0.523 0.866 1.469 2.013 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.14 0.21 0.311 0.523 0.866 1.469 2.013 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.14 0.21 0.311 0.523 0.866 1.469 2.013 O.D. 0.052 0.14 0.21 0.311 0.523 0.866 1.469 2.013
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
