- Catalog number
- EK0926
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead, the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 27 30 28 28 1.08 3.8% Sample 2 81 79 81 73 78 3.27 4.1% Sample 3 453 479 435 456 455 15.64 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 27 30 28 28 1.08 3.8% Sample 1 28 27 30 28 28 1.08 3.8% Sample 2 81 79 81 73 78 3.27 4.1% Sample 2 81 79 81 73 78 3.27 4.1% Sample 3 453 479 435 456 455 15.64 3.4% Sample 3 453 479 435 456 455 15.64 3.4%
- Synonyms
- Natural killer cell receptor 2B4; NK cell activation-inducing ligand; NAIL; NK cell type I receptor protein 2B4; NKR2B4; h2B4; SLAM family member 4; SLAMF4; Signaling lymphocytic activation molecule 4; CD244; CD244;2B4
- Gene Name
- CD244
- Gene ID
- 51744
- UniProt
- Q9BZW8
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: C22-R221
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils. .
- Applications
- ELISA
- Field of Research
- Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, NK Cells, Stem Cells, T Lymphocytic Lineage
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 81 453 30 112 469 Standard deviation 1.34 4.62 20.84 1.53 6.61 30.02 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 81 453 30 112 469 Standard deviation 1.34 4.62 20.84 1.53 6.61 30.02 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 81 453 30 112 469 Mean (pg/ml) 28 81 453 30 112 469 Standard deviation 1.34 4.62 20.84 1.53 6.61 30.02 Standard deviation 1.34 4.62 20.84 1.53 6.61 30.02 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4%
- Sensitivity
- <10 pg/mL
- Function
- Modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 41616 MW
- Specificity
- Natural and recombinant human CD244
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Natural killer cell receptor 2B4
- Subcellular Location
- Membrane ; Single-pass type I membrane protein .
- Protein Name
- Natural killer cell receptor 2B4
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.087 0.134 0.235 0.409 0.722 1.329 2.21 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.087 0.134 0.235 0.409 0.722 1.329 2.21 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.087 0.134 0.235 0.409 0.722 1.329 2.21 O.D. 0.032 0.087 0.134 0.235 0.409 0.722 1.329 2.21
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
