- Catalog number
- EK0928
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 31 27 28 1.47 5.2% Sample 2 204 231 201 203 209 12.31 5.8% Sample 3 455 425 389 431 425 23.62 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 31 27 28 1.47 5.2% Sample 1 28 29 31 27 28 1.47 5.2% Sample 2 204 231 201 203 209 12.31 5.8% Sample 2 204 231 201 203 209 12.31 5.8% Sample 3 455 425 389 431 425 23.62 5.5% Sample 3 455 425 389 431 425 23.62 5.5%
- Synonyms
- Tissue factor; TF; Coagulation factor III; Thromboplastin; CD142; F3
- Gene Name
- F3
- Gene ID
- 2152
- UniProt
- P13726
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S33-E251
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Lung, placenta and pancreas. .
- Applications
- ELISA
- Field of Research
- Cardiovascular
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA, citrate), Cell lysates, and urine.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 204 455 28 205 493 Standard deviation 1.26 13.46 34.58 1.54 14.14 40.91 CV (%) 4.5% 6.6% 7.6% 5.5% 6.9% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 204 455 28 205 493 Standard deviation 1.26 13.46 34.58 1.54 14.14 40.91 CV (%) 4.5% 6.6% 7.6% 5.5% 6.9% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 204 455 28 205 493 Mean (pg/ml) 28 204 455 28 205 493 Standard deviation 1.26 13.46 34.58 1.54 14.14 40.91 Standard deviation 1.26 13.46 34.58 1.54 14.14 40.91 CV (%) 4.5% 6.6% 7.6% 5.5% 6.9% 8.3% CV (%) 4.5% 6.6% 7.6% 5.5% 6.9% 8.3%
- Sensitivity
- <10 pg/mL
- Function
- Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 33068 MW
- Specificity
- Natural and recombinant human Tissue factor
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Tissue factor
- Subcellular Location
- Isoform 1: Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Belongs to the tissue factor family.
- Protein Name
- Tissue factor
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.055 0.087 0.157 0.304 0.57 1.145 2.113 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.055 0.087 0.157 0.304 0.57 1.145 2.113 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.055 0.087 0.157 0.304 0.57 1.145 2.113 O.D. 0.034 0.055 0.087 0.157 0.304 0.57 1.145 2.113
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
