- Catalog number
- EK0932
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-12 (IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF.2 Both IL-12 p40 (-/-) and p35 (-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin (IL) -12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN) -gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 28 30 33 31 2.12 6.8% Sample 2 233 231 232 259 238 11.71 4.9% Sample 3 425 425 444 461 438 15 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 28 30 33 31 2.12 6.8% Sample 1 33 28 30 33 31 2.12 6.8% Sample 2 233 231 232 259 238 11.71 4.9% Sample 2 233 231 232 259 238 11.71 4.9% Sample 3 425 425 444 461 438 15 3.4% Sample 3 425 425 444 461 438 15 3.4%
- Synonyms
- Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; Il12b
- Gene Name
- IL12B
- Gene ID
- 16160
- UniProt
- P43432
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: M23-S335
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Macrophage/Inflammation, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 233 425 35 253 463 Standard deviation 1.84 13.98 22.52 1.96 17.45 29.63 CV (%) 6% 6% 5.3% 5.6% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 233 425 35 253 463 Standard deviation 1.84 13.98 22.52 1.96 17.45 29.63 CV (%) 6% 6% 5.3% 5.6% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 233 425 35 253 463 Mean (pg/ml) 33 233 425 35 253 463 Standard deviation 1.84 13.98 22.52 1.96 17.45 29.63 Standard deviation 1.84 13.98 22.52 1.96 17.45 29.63 CV (%) 6% 6% 5.3% 5.6% 6.9% 6.4% CV (%) 6% 6% 5.3% 5.6% 6.9% 6.4%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 38235 MW
- Specificity
- Natural and recombinant mouse IL-12 (p40)
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Interleukin-12 subunit beta
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the type I cytokine receptor family. Type 3 subfamily.
- Protein Name
- Interleukin-12 subunit beta
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.093 0.158 0.271 0.504 0.878 1.344 1.938 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.093 0.158 0.271 0.504 0.878 1.344 1.938 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.093 0.158 0.271 0.504 0.878 1.344 1.938 O.D. 0.023 0.093 0.158 0.271 0.504 0.878 1.344 1.938
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
