Human IL-22/Interleukin-22 ELISA Kit PicoKine®

About this product

Human IL-22 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0933

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 33 38 33 36 3.08 8.5% Sample 2 215 247 244 248 238 13.64 5.7% Sample 3 435 437 429 427 432 4.12 0.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 33 38 33 36 3.08 8.5% Sample 1 40 33 38 33 36 3.08 8.5% Sample 2 215 247 244 248 238 13.64 5.7% Sample 2 215 247 244 248 238 13.64 5.7% Sample 3 435 437 429 427 432 4.12 0.9% Sample 3 435 437 429 427 432 4.12 0.9%

Synonyms

Interleukin-22; IL-22; Cytokine Zcyto18; IL-10-related T-cell-derived-inducible factor; IL-TIF; IL22; ILTIF, ZCYTO18; UNQ3099/PRO10096

Gene Name

IL22

Gene ID

50616

UniProt

Q9GZX6

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A34-I179

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adapters, Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation, Signal Transduction, Transmembrane

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 215 435 41 220 427 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 CV (%) 6.4% 6.9% 6.2% 8.6% 8.6% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 215 435 41 220 427 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 CV (%) 6.4% 6.9% 6.2% 8.6% 8.6% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 215 435 41 220 427 Mean (pg/ml) 40 215 435 41 220 427 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 CV (%) 6.4% 6.9% 6.2% 8.6% 8.6% 6.4% CV (%) 6.4% 6.9% 6.2% 8.6% 8.6% 6.4%

Sensitivity

<10 pg/mL

Function

Cytokine that contributes to the inflammatory response in vivo.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20011 MW

Specificity

Natural and recombinant human IL-22

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-22

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-10 family.

Protein Name

Interleukin-22

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.112 0.226 0.37 0.643 1.114 1.566 1.925 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.112 0.226 0.37 0.643 1.114 1.566 1.925 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.112 0.226 0.37 0.643 1.114 1.566 1.925 O.D. 0.016 0.112 0.226 0.37 0.643 1.114 1.566 1.925

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.