- Catalog number
- EK0936
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM) .3 A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 345 375 350 321 347 19.17 5.5% Sample 2 1737 1912 2076 1952 1919 121.35 6.3% Sample 3 4428 3889 4055 4258 4157 203.63 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 345 375 350 321 347 19.17 5.5% Sample 1 345 375 350 321 347 19.17 5.5% Sample 2 1737 1912 2076 1952 1919 121.35 6.3% Sample 2 1737 1912 2076 1952 1919 121.35 6.3% Sample 3 4428 3889 4055 4258 4157 203.63 4.8% Sample 3 4428 3889 4055 4258 4157 203.63 4.8%
- Synonyms
- E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD62E; Sele; Elam-1
- Gene Name
- SELE
- Gene ID
- 25544
- UniProt
- P98105
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: W22-P494
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cofactors, Vitamins/Minerals, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oncoproteins/Suppressors, Pathways and Processes, Signal Transduction, Tumor Suppressors, Vitamins/Minerals
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 345 1737 4428 315 1621 4077 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 345 1737 4428 315 1621 4077 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 345 1737 4428 315 1621 4077 Mean (pg/ml) 345 1737 4428 315 1621 4077 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6% CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6%
- Sensitivity
- <10 pg/mL
- Function
- Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 60080 MW
- Specificity
- Natural and recombinant rat sE-Selectin
- Anticoagulant
- Heparin or citrate
- Gene Name Synonym
- E-selectin
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein.
- Sequence Similarities
- Belongs to the selectin/LECAM family.
- Protein Name
- E-selectin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.086 0.174 0.331 0.637 1.052 1.614 2.2 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.086 0.174 0.331 0.637 1.052 1.614 2.2 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.086 0.174 0.331 0.637 1.052 1.614 2.2 O.D. 0.008 0.086 0.174 0.331 0.637 1.052 1.614 2.2
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
