Rat sL-Selectin ELISA Kit PicoKine®

About this product

Rat sL-Selectin ELISA Kit PicoKine® (96 Tests) . Quantitate Rat SELL in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0937

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a homing receptor for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1474 1304 1342 1320 1360 67.18 4.9% Sample 2 3614 3452 3351 3314 3432 116.19 3.3% Sample 3 24600 22997 23584 22075 23314 916.82 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1474 1304 1342 1320 1360 67.18 4.9% Sample 1 1474 1304 1342 1320 1360 67.18 4.9% Sample 2 3614 3452 3351 3314 3432 116.19 3.3% Sample 2 3614 3452 3351 3314 3432 116.19 3.3% Sample 3 24600 22997 23584 22075 23314 916.82 3.9% Sample 3 24600 22997 23584 22075 23314 916.82 3.9%

Synonyms

L-selectin ; Sell ; rCG_45999

Gene Name

SELL

Gene ID

29259

UniProt

Q63762

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: W39-N332

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, Cardiovascular, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells, T Cells, T Lymphocytic Lineage

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1474 3614 24600 1588 3716 23760 Standard deviation 89.91 166.24 1279.2 100.04 178.36 1663.2 CV (%) 6.1% 4.6% 5.2% 4.8%% 4.8% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1474 3614 24600 1588 3716 23760 Standard deviation 89.91 166.24 1279.2 100.04 178.36 1663.2 CV (%) 6.1% 4.6% 5.2% 4.8%% 4.8% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1474 3614 24600 1588 3716 23760 Mean (pg/ml) 1474 3614 24600 1588 3716 23760 Standard deviation 89.91 166.24 1279.2 100.04 178.36 1663.2 Standard deviation 89.91 166.24 1279.2 100.04 178.36 1663.2 CV (%) 6.1% 4.6% 5.2% 4.8%% 4.8% 7% CV (%) 6.1% 4.6% 5.2% 4.8%% 4.8% 7%

Sensitivity

<300 pg/mL

Function

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

42471 MW

Specificity

Natural and recombinant rat sL-Selectin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

L-selectin

Sequence Similarities

Belongs to the selectin/LECAM family.

Protein Name

L-selectin

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.